CRYSTAL-STRUCTURE OF CORE STREPTAVIDIN DETERMINED FROM MULTIWAVELENGTH ANOMALOUS DIFFRACTION OF SYNCHROTRON RADIATION
CRYSTAL-STRUCTURE OF CORE STREPTAVIDIN DETERMINED FROM MULTIWAVELENGTH ANOMALOUS DIFFRACTION OF SYNCHROTRON RADIATION
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DOI：
10.1073/pnas.86.7.2190
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
PHIZACKERLEY, RP
中科院分区：
文献类型：
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作者：
HENDRICKSON, WA;PAHLER, A;PHIZACKERLEY, RP
链霉亲和素结合核心的三维晶体结构在3.1 . ang处被确定。决议。通过对硒生物素与链霉亲和素配合物单晶在三个波长下的衍射数据分析了其结构。链霉亲和素是一种四聚体，其亚基以D2对称排列。每个原聚体是一个8链。-桶具有简单的上下拓扑结构。生物素分子与每个桶中的一个结合在一起。本研究证明了多波长异常衍射（MAD）方法在大分子晶体学中的有效性，并为生物素-亲和素相互作用的详细研究提供了基础。
A three-dimensional crystal structure of the biotin-binding core of streptavidin has been determined at 3.1 .ANG. resolution. The structure was analyzed from diffraction data measured at three wavelengths from a single crystal of the selenobiotinyl complex with streptavidin. Streptavidin is a tetramer with subunits arrayed in D2 symmetry. Each protomer is an 8-stranded .beta.-barrel with simple up-down topology. Biotin molecules are bound at one of each barrel. This study demonstrates the effectiveness of multiwavelength anomalous diffraction (MAD) procedures for macromolecular crystallography and provides a basis for detailed study of biotin-avidin interactions.