Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells.
Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells.
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DOI：
10.1038/srep36120
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发表时间：
2016-10-27
影响因子：
4.6
通讯作者：
Lerman LO
中科院分区：
文献类型：
--
作者：
Eirin A;Zhu XY;Puranik AS;Woollard JR;Tang H;Dasari S;Lerman A;van Wijnen AJ;Lerman LO
从间充质干细胞/基质细胞（MSC）分离的细胞外囊泡（EV）有助于受损组织的恢复。我们先前已经表明，猪MSC衍生的EV转运能够调节受体细胞中的细胞通路的mRNA和miRNA。为了确定有助于猪MSC衍生EV的治疗效果的候选因素，我们使用蛋白质组学表征了它们的蛋白质货物。从腹部脂肪培养猪MSC，并且EV表征典型MSC和EV标志物的表达。进行LC-MS/MS蛋白质组学分析并对蛋白质进行分类。进行了功能途径分析，并通过蛋白质印迹法验证了五种候选蛋白。蛋白质组学分析在MSC中鉴定了5，469种不同的蛋白质，在EV中鉴定了4，937种。平均蛋白质表达在MSC中高于EV。差异表达分析揭示了128种蛋白质选择性地富集在EV与MSC中，而563种蛋白质从EV中排除。EV中富集的蛋白质与广泛的生物学功能相关，包括血管生成、血液凝固、细胞凋亡、细胞外基质重塑和炎症调节。排除的主要是核蛋白，如参与核苷酸结合和RNA剪接的蛋白质。EV具有选择性富集的蛋白质货物，其具有特定的生物特征，MSC可以用于细胞间通讯以促进组织修复。
Extracellular vesicles (EVs) isolated from mesenchymal stem/stromal cells (MSCs) contribute to recovery of damaged tissue. We have previously shown that porcine MSC-derived EVs transport mRNA and miRNA capable of modulating cellular pathways in recipient cells. To identify candidate factors that contribute to the therapeutic effects of porcine MSC-derived EVs, we characterized their protein cargo using proteomics. Porcine MSCs were cultured from abdominal fat, and EVs characterized for expression of typical MSC and EV markers. LC-MS/MS proteomic analysis was performed and proteins classified. Functional pathway analysis was performed and five candidate proteins were validated by western blot. Proteomics analysis identified 5,469 distinct proteins in MSCs and 4,937 in EVs. The average protein expression was higher in MSCs vs. EVs. Differential expression analysis revealed 128 proteins that are selectively enriched in EVs versus MSCs, whereas 563 proteins were excluded from EVs. Proteins enriched in EVs are linked to a broad range of biological functions, including angiogenesis, blood coagulation, apoptosis, extracellular matrix remodeling, and regulation of inflammation. Excluded are mostly nuclear proteins, like proteins involved in nucleotide binding and RNA splicing. EVs have a selectively-enriched protein cargo with a specific biological signature that MSCs may employ for intercellular communication to facilitate tissue repair.
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影响因子：
3.3
作者：
Eirin A;Zhu XY;Ebrahimi B;Krier JD;Riester SM;van Wijnen AJ;Lerman A;Lerman LO
通讯作者：
Lerman LO
影响因子：
11.8
作者：
Castets, Marie;Coissieux, Marie-May;Mehlen, Patrick
通讯作者：
Mehlen, Patrick
影响因子：
3.7
作者：
Ebrahimi B;Eirin A;Li Z;Zhu XY;Zhang X;Lerman A;Textor SC;Lerman LO
通讯作者：
Lerman LO
人骨髓间充质干细胞来源的细胞外囊泡促进大鼠心肌梗死模型中的血管生成
影响因子：
4.7
作者：
Bian, Suyan;Zhang, Liping;Yu, Hepeng
通讯作者：
Yu, Hepeng
影响因子：
5.2
作者：
Eirin, Alfonso;Zhu, Xiang-Yang;Krier, James D.;Tang, Hui;Jordan, Kyra L.;Grande, Joseph P.;Lerman, Amir;Textor, Stephen C.;Lerman, Lilach O.
通讯作者：
Lerman, Lilach O.