Transcriptome profiling of bovine ovarian theca cells treated with fibroblast growth factor 9.
Transcriptome profiling of bovine ovarian theca cells treated with fibroblast growth factor 9.
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DOI：
10.1016/j.domaniend.2017.12.002
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发表时间：
2018-04
影响因子：
2.1
通讯作者：
Spicer LJ
中科院分区：
文献类型：
--
作者：
Schütz LF;Hurst RE;Schreiber NB;Spicer LJ
我们以前报道过成纤维细胞生长因子9(FGF9)作为一种抗分化因子，刺激颗粒细胞(GC)和卵泡膜细胞(TC)的增殖，同时抑制激素诱导的类固醇合成。FGF9是如何同时抑制类固醇合成和刺激增殖的，目前还没有完全阐明。因此，本研究旨在阐明FGF9对TC转录组的影响。取自当地屠宰场的小母牛卵巢，从大的有腔卵泡分离TC，分别加入或不加入30 ng/mLFGF9和IGF-1培养24 h。处理后，从TC中提取总RNA，用Affymetrix基因芯片牛基因组阵列(n=4/组)处理成微阵列。用1.3倍截止值和P<；0.05显著水平比较FGF9处理的TC和对照TC，转录组分析鉴定出355个差异表达的转录本，其中164个元件上调，191个元件下调。利用独创性通路分析(IPA)研究了FGF9处理如何影响牛TC的分子通路、生物学功能和分子间的联系。IPA软件确定了与TC中FGF9相关的346条途径，涉及多种生物学功能，并揭示了与细胞增殖相关的基因(如CCND1、FZD5和MYB)、抗氧化/细胞保护基因(如Hmox1和NQO1)和类固醇合成基因(如Cyp11a1和STAR)之间的有趣关系。总之，本研究中确定的基因、途径和网络描绘了FGF9如何调控卵泡发生的图景，为进一步研究FGF9在卵巢卵泡发育中的功能提供了新的候选基因。
We reported previously that fibroblast growth factor 9 (FGF9) acts as an anti-differentiation factor, stimulating proliferation of granulosa (GC) and theca (TC) cells while suppressing hormone-induced steroidogenesis of these cells. How FGF9 acts to simultaneously suppress steroidogenesis and stimulate proliferation remains to be fully elucidated. Thus, this study was undertaken to clarify the effects of FGF9 on the TC transcriptome. Ovaries were obtained from beef heifers at a local abattoir, TC were isolated from large antral follicles, and cultured with or without 30 ng/mL of FGF9 for 24 h in the presence of LH and IGF-1. Following treatment, total RNA was extracted from TC and processed for microarray using Affymetrix GeneChip Bovine Genome Arrays (n = 4/group). Transcriptome analysis comparing FGF9-treated TC with control TC using 1.3-fold cut-off and a P < 0.05 significance level identified 355 differentially expressed transcripts, with 164 elements up-regulated and 191 elements down-regulated by FGF9. The Ingenuity Pathway Analysis (IPA) was utilized to investigate how FGF9 treatment affects molecular pathways, biological functions, and the connection between molecules in bovine TC. The IPA software identified 346 pathways in response to FGF9 in TC involved in several biological functions, and unveiled interesting relationships among genes related to cell proliferation (e.g., CCND1, FZD5 and MYB), antioxidation/cytoprotection (e.g., HMOX1 and NQO1) and steroidogenesis (e.g., CYP11A1 and STAR). Overall, genes, pathways, and networks identified in this study painted a picture of how FGF9 may regulate folliculogenesis, providing novel candidate genes for further investigation of FGF9 functions in ovarian follicular development.
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影响因子：
11.2
作者：
Hendrix, ND;Wu, R;Cho, KR
通讯作者：
Cho, KR
影响因子：
2.1
作者：
Dentis, J. L.;Schreiber, N. B.;Spicer, L. J.
通讯作者：
Spicer, L. J.
影响因子：
3.3
作者：
Grado-Ahuir, J. A.;Aad, P. Y.;Spicer, L. J.
通讯作者：
Spicer, L. J.
影响因子：
64.5
作者：
Colvin, JS;Green, RP;Ornitz, DM
通讯作者：
Ornitz, DM
影响因子：
3.6
作者：
Boone, DL;Tsang, BK
通讯作者：
Tsang, BK