Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1
Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1
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Dicer 敲除通过诱导 Egr1 诱导 HEK293T 细胞中纤连蛋白-1 的表达。
DOI：
10.1016/j.bbagen.2009.11.009
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发表时间：
2010-03-01
影响因子：
3
通讯作者：
Li, Yong-Hua
中科院分区：
文献类型：
--
作者：
Tang, Kai-Fu;Song, Guan-Bin;Li, Yong-Hua
背景：Dicer 是一种多域核糖核酸酶 III，参与 microRNA (miRNA) 和小干扰 RNA (siRNA) 的生物发生；发现 Dicer 的缺失会损害内皮细胞的迁移。方法：siRNA 转染、细胞迁移测定、实时 RT-PCR、染色质免疫沉淀、Western blotting、ELISA、caspase-3 活性测定和annexin-V-FITC 测定。结果：Dicer 的敲低损害了 HEK293T 细胞的迁移能力并诱导纤连蛋白-1。纤连蛋白-1 的上调依赖于 Egr1。与纤连蛋白-1单敲低细胞相比，纤连蛋白-1/Dicer双敲低细胞的细胞凋亡显着增加。结论：Dicer表达减少通过Egr1依赖性方式诱导纤连蛋白-1表达。一般意义：我们的数据表明纤连蛋白-1的上调可保护Dicer敲低HEK293T细胞免于凋亡。 (C) 2009 Elsevier B.V. 保留所有权利。
Background: Dicer is a multidomain ribonuclease III enzyme involved in the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs); depletion of Dicer was found to impair the migration of endothelial cells.Methods: siRNA transfection, cell migration assay, real-time RT-PCR, chromatin immunoprecipitation, Western blotting, ELISA, caspase-3 activity assay, and annexin-V-FITC assay were utilized.Results: Knockdown of Dicer impairs the migratory capacity of HEK293T cells and induces fibronectin-1. The upregulation of fibronectin-1 is dependent on Egr1. Fibronectin-1/Dicer double-knockdown cells showed a marked increase in apoptosis compared with fibronectin-1 single knockdown cells.Conclusions: Decreased Dicer expression induces fibronectin-1 expression via an Egr1-dependent manner.General significance: Our data suggest that upregulation of fibronectin-1 protects Dicer knockdown HEK293T cells against apoptosis. (C) 2009 Elsevier B.V. All rights reserved.