Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling.
Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling.
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DOI：
10.1016/j.immuni.2010.07.015
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发表时间：
2010-08-27
期刊：
影响因子：
32.4
通讯作者：
Tangye SG
中科院分区：
文献类型：
--
作者：
Deenick EK;Chan A;Ma CS;Gatto D;Schwartzberg PL;Brink R;Tangye SG
有效的体液免疫依赖于T-滤泡辅助（Tfh）细胞对B细胞应答的支持。虽然已经提出Tfh细胞分化需要T-B相互作用，但Ag呈递细胞的特定群体的相对贡献仍然未知。我们采用了三种独立的策略，在体内损害CD 4 + T细胞和活化的B细胞之间的相互作用。而CD 4 + T细胞的扩增相对不受影响，Tfh细胞分化在所有情况下都被完全阻断。令人惊讶的是，增强非B细胞的抗原呈递挽救了Tfh细胞分化，如通过表面表型、基因表达和生发中心定位所确定的。我们的结论是，虽然抗原呈递的应答B细胞通常需要的Tfh细胞的产生，这并不导致从提供一个独特的B细胞衍生的信号，而是因为应答B细胞迅速成为抗原的主要来源。
Effective humoral immunity depends on the support of B cell responses by T-follicular helper (Tfh) cells. Whilst it has been proposed that Tfh cell differentiation requires T-B interactions, the relative contribution of specific populations of Ag presenting cells remains unknown. We employed three independent strategies that compromised interactions between CD4+ T cells and activated B cells in vivo. Whereas the expansion of CD4+ T cells was relatively unaffected, Tfh cell differentiation was completely blocked in all scenarios. Surprisingly, augmenting antigen presentation by non-B cells rescued Tfh cell differentiation, as determined by surface phenotype, gene expression and germinal center localization. We conclude that although Ag presentation by responding B cells is typically required for the generation of Tfh cells, this does not result from the provision of a unique B cell-derived signal, but rather because responding B cells rapidly become the primary source of antigen.
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发表时间：
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期刊：
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