Succinic Acid Production by Actinobacillus succinogenes Using Spent Brewer's Yeast Hydrolysate as a Nitrogen Source
Succinic Acid Production by Actinobacillus succinogenes Using Spent Brewer's Yeast Hydrolysate as a Nitrogen Source
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使用啤酒废酵母水解物作为氮源由产琥珀酸放线杆菌生产琥珀酸
DOI：
10.1007/s12010-009-8649-1
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Chang, Honam
中科院分区：
文献类型：
--
作者：
Jiang, Min;Chen, Kequan;Chang, Honam
为了开发一种经济有效的发酵培养基，对废啤酒酵母水解物作为含葡萄糖培养基中产琥珀酸放线杆菌 NJ113 生产琥珀酸的氮源进行了评估。使用自溶和酶水解来水解废啤酒酵母细胞以释放营养物质。结果表明，酶水解是一种更有效的方法，因为它具有更高的琥珀酸产量和细胞生长。然而，葡萄糖消耗不完全表明存在营养限制。随后，维生素被确定为使用酶水解废啤酒酵母作为氮源生产琥珀酸的主要限制因素。添加维生素后，细胞生长和琥珀酸浓度均得到改善。结果，在补充维生素的情况下，可以成功地用啤酒废酵母的酶解产物替代15 g/L的酵母提取物，从而从68 g/L的葡萄糖生产46.8 g/L的琥珀酸。
To develop a cost-effective fermentation medium, spent brewer's yeast hydrolysate was evaluated as a nitrogen source for succinic acid production by Actinobacillus succinogenes NJ113 in glucose-containing media. Autolysis and enzymatic hydrolysis were used to hydrolyze the spent brewer's yeast cells to release the nutrients. The results showed that enzymatic hydrolysis was a more effective method due to the higher succinic acid yield and cell growth. However, the incomplete glucose consumption indicated existence of nutrient limitation. Vitamins were subsequently identified as the main limiting factors for succinic acid production using enzymatically hydrolyzed spent brewer's yeast as a nitrogen source. After the addition of vitamins, cell growth and succinic acid concentration both improved. As a result, 15 g/L yeast extract could be successfully replaced with the enzymatic hydrolysate of spent brewer's yeast with vitamins supplementation, resulting in a production of 46.8 g/L succinic acid from 68 g/L glucose.