Measurement of antibodies to gastrointestinal nematodes and liver fluke in meat juice of beef cattle and associations with carcass parameters
Measurement of antibodies to gastrointestinal nematodes and liver fluke in meat juice of beef cattle and associations with carcass parameters
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DOI：
10.1016/j.vetpar.2009.09.040
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发表时间：
2009-12-23
影响因子：
2.6
通讯作者：
Vercruysse, Jozef
中科院分区：
文献类型：
--
作者：
Charlier, Johannes;De Cat, Ann;Vercruysse, Jozef
为了开发无创诊断牛肝片形吸虫感染的方法，本研究对肉汁样品中的抗体检测elisa进行了评估，并探讨了检测结果与胴体参数之间的关系。为确定肉汁样品的最佳工作稀释度，进行了初步试验。90 ~ 100头奶牛血清和肉汁样品的ELISA检测结果（以ODR表示）的Pearson相关系数分别为R = 0.82和0.75。接下来，对比利时蓝乳牛进行了屠宰场调查，分析了2008年春季726头奶牛和秋季724头奶牛的肉汁样本，这些奶牛来自总共480个畜群。黄鳝和黄鳝的odr值存在较大差异，黄鳝的odr值存在季节间差异（春季、秋季），黄鳝的odr值不存在季节间差异。采用线性或logistic混合模型研究个体寄生虫特异性酶联免疫吸附试验结果与胴体参数（温胴体重、构象评分、脂肪覆盖率）之间的关系，采用随机效应模型研究群体平均酶联免疫吸附试验结果与胴体平均体重之间的关系，以平均牛龄和季节为协变量，采用线性回归方法研究群体平均胴体重与群体平均胴体重之间的关系。ostertagi个体ODR在四分位数范围内的增加与低构象评分（得分E比S）的可能性增加1.3相关，但与其他胴体参数没有显著相关性。然而，群体平均酶联免疫吸附试验结果与群体平均胴体重的降低呈负相关（ostertagi和F.肝分别为P = 0.001和P = 0.09）。在四分位数范围内，猪群平均ostertagi或F.肝炎ODR的增加分别导致猪群平均胴体重减少9.1 kg和3.4 kg。讨论了这些结果的意义和通过分析屠宰场肉汁样品来监测寄生虫感染的价值。(C) 2009 Elsevier B.V.版权所有
In an effort to develop non-invasive methods for the diagnosis of Ostertagia ostertagi and Fasciola hepatica infection in beef cattle, this study was undertaken to evaluate antibody-detection ELISAs in meat juice samples and to investigate the associations between test results and carcass parameters. Preliminary tests were carried out to determine optimal working dilutions of meat juice samples. The Pearson correlation coefficients between ELISA results (expressed as ODR) of serum and meat juice samples from 90 to 100 cows were R = 0.82 and 0.75 for O. ostertagi and F. hepatica, respectively. Next, an abattoir survey in Belgian Blue suckler cows was performed, analysing meat juice samples from 726 animals in spring and 724 animals in autumn 2008, originating from a total of 480 herds. There was a large variation in the observed O. ostertagi and F. hepatica ODRs and inter-seasonal differences were observed for F. hepatica (spring > autumn), but not for O. ostertagi. The relationships between individual parasite-specific ELISA results and carcass parameters (warm carcass weight, conformation score, fat coverage) were investigated by linear or logistic mixed models with herd as a random effect, while the relationship between herd average ELISA results and herd averaged carcass weight was investigated by linear regression with mean cow age and season as covariates. An increase in individual O. ostertagi ODR over the interquartile range was associated with an increased likelihood by 1.3 of a low conformation score (score E vs. S), but no significant associations were found with other carcass parameters. However, herd mean ELISA results were negatively associated with a decrease in the herd average carcass weight (P = 0.001 and P = 0.09 for O. ostertagi and F. hepatica, respectively). An increase in the herd mean O. ostertagi or F. hepatica ODR over the interquartile range was associated with a decrease in herd mean carcass weight of 9.1 and 3.4 kg, respectively. The implications of these results and the value of monitoring parasitic infections by analysis of meat juice samples from the abattoir are discussed. (C) 2009 Elsevier B.V. All rights reserved.