LOCOMOTION OF MOUSE FIBROBLASTS IN TISSUE CULTURE
LOCOMOTION OF MOUSE FIBROBLASTS IN TISSUE CULTURE
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DOI：
10.1016/s0006-3495(70)86347-0
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发表时间：
1970-01-01
影响因子：
3.4
通讯作者：
BOONE, CW
中科院分区：
文献类型：
--
作者：
GAIL, MH;BOONE, CW
采用延时摄影技术研究了小鼠成纤维细胞在组织培养中的运动。在连续的短时间间隔的观察揭示了细胞的趋势，坚持在其运动方向，从一个2.5小时的时间间隔到下一个。然而，超过5.0小时的时间间隔，运动的方向似乎是随机的。这一事实表明，如果使用适当长的观察时间，D，随机游走模型的扩散常数，可能有助于表征细胞运动。因此，我们研究了“持久性”对纯随机游走模型的影响，我们从理论上发现并通过实验证实，持久性细胞的运动性确实可以通过增强的扩散常数D* 来表征。还提出了确定D ~* 置信限的方法。因此，一个随机游走模型，修改理解的持久性效应，被发现来描述在组织培养中的成纤维细胞的运动，并提供一个细胞运动的数值测量。
Time-lapse cinematography was used to investigate the motion of mouse fibroblasts in tissue culture. Observations over successive short time intervals revealed a tendency for the cells to persist in their direction of motion from one 2.5 hr time interval to the next. Over 5.0-hr time intervals, however, the direction of motion appeared random. This fact suggested thatD, the diffusion constant of a random walk model, might serve to characterize cellular motility if suitably long observation times were used. We therefore investigated the effect of "persistence" on the pure random walk model, and we found theoretically and confirmed experimentally that the motility of a persisting cell could indeed be characterized by an augmented diffusion constant,D*. A method for determining confidence limits onD* was also developed. Thus a random walk model, modified to comprehend the persistence effect, was found to describe the motion of fibroblasts in tissue culture and to provide a numerical measure of cellular motility.