Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat
Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat
复制标题
DOI：
10.1369/0022155419859861
复制
发表时间：
2019-06
影响因子：
3.2
通讯作者：
K. Kami;T. Ohira;Y. Oishi;T. Nakajima;K. Goto;Y. Ohira
中科院分区：
文献类型：
--
作者：
K. Kami;T. Ohira;Y. Oishi;T. Nakajima;K. Goto;Y. Ohira
间歇性热应激有助于受伤肌肉的再生。热应激会增加72 kda热休克蛋白（HSP72）的水平，可能与这种效应有关，但其确切机制尚不清楚。本研究采用免疫组织化学方法研究热休克蛋白72在热应激大鼠再生肌肉中的定位及其作用。在比目鱼肌注射布比卡因后，将大鼠下体浸泡在热水中（42℃，30分钟，每隔一天），施加热应激。1周后，我们发现HSP72不仅在存活的肌纤维中高水平表达，而且在加热大鼠再生肌肉的血管中也有高水平表达。此外，再生肌纤维周围毛细血管内表达分化簇43的白细胞（可能是粒细胞）也高度表达HSP72。相比之下，HSP72在无热应激的完整或再生肌肉中未见明显表达。这些结果表明，热应激诱导的肌纤维、血管和循环白细胞中的HSP72可能在促进热应激损伤肌肉的再生中发挥重要作用。我们的发现将有助于研究HSP72在骨骼肌再生过程中的细胞特异性作用。
The regeneration of injured muscles is facilitated by intermittent heat stress. The 72-kDa heat shock protein (HSP72), the level of which is increased by heat stress, is likely involved in this effect, but the precise mechanism remains unclear. This study was conducted to investigate the localization and role(s) of HSP72 in the regenerating muscles in heat-stressed rats using immunohistochemistry. Heat stress was applied by immersion of the rat lower body into hot water (42C, 30 min, every other day) following injection of bupivacaine into the soleus muscles. After 1 week, we found that HSP72 was expressed at high levels not only in the surviving myofibers but also in the blood vessels of the regenerating muscles in heated rats. In addition, leukocytes, possibly granulocytes, expressing cluster of differentiation 43 within the blood capillaries surrounding the regenerating myofibers also highly expressed HSP72. In contrast, marked expression of HSP72 was not observed in the intact or regenerating muscles without heat stress. These results suggest that heat-stress-induced HSP72 within the myofibers, blood vessels, and circulating leukocytes may play important roles in enhancing regeneration of injured muscles by heat stress. Our findings would be useful to investigate cell-specific role(s) of HSP72 during skeletal muscle regeneration.