Analysis of fluorotelomer alcohols, fluorotelorner acids, and short- and long-chain perfluorinated acids in water and biota
Analysis of fluorotelomer alcohols, fluorotelorner acids, and short- and long-chain perfluorinated acids in water and biota
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DOI：
10.1016/j.chroma.2005.07.053
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发表时间：
2005-11-04
影响因子：
4.1
通讯作者：
Yamashita, N
中科院分区：
文献类型：
--
作者：
Taniyasu, S;Kannan, K;Yamashita, N
含氟调质体醇和含氟调质体酸已被认为是在偏远海洋地区发现的全氟化羧酸的来源。为了研究环境中全氟酸的来源和去向，需要一种测定环境和生物基质中多种多氟酸和全氟酸的方法。在这项研究中，建立了一种测定水和生物样品中一系列中性和酸性含氟化合物的方法，包括含氟调聚体醇、含氟调聚体酸和短链和长链全氟酸。该方法包括用弱阴离子交换(蜡柱)固相萃取，然后用醋酸钠缓冲液、甲醇和0.1%NH4OH在甲醇中依次洗脱。对于生物样品，在固相萃取之前，组织在0.5N氢氧化钾/甲醇中消化，在水中稀释，然后通过蜡盒。中性化合物和端粒醇与其他多氟酸和全氟酸分开。该方法可靠(即能够测量中性和酸性化合物)，可用于水和生物群中的一系列多氟酸和全氟酸的分析，包括端粒醇、端粒酸、全氟烷基羧酸盐和全氟烷基磺酸盐。利用高效液相色谱-串联质谱仪(HPLCMS/MS)，水中的检出限在千分之几十到几百(pg/L)之间，生物基质中的检出限在万亿分之几十到几百(pg/g)之间。(C)2005 Elsevier B.V.保留所有权利。
Fluorotelomer alcohols and fluorotelomer acids have been proposed as a source of the perfluorinated carboxylic acids found in remote marine locations. To examine the sources and fate of perfluorinated acids in the environment, a method to determine a wide range of poly- and perfluorinated acids in environmental and biological matrices is needed. In this study, a method has been developed to measure a suite of neutral and acidic fluorochemicals including, fluorotelomer alcohols, fluorotelomer acids, and short- and long-chain perfluorinated acids, in water and biological samples. The method involves solid-phase extraction with weak anion exchange (WAX) cartridges, followed by sequential elution with sodium acetate buffer, methanol, and 0.1% NH4OH in methanol. For biological samples, prior to solid-phase extraction, tissues are digested in 0.5N potassium hydroxide/methanol, diluted in water, and passed through the WAX cartridge. Neutral compounds and telomer alcohols are separated from other poly- and perfluorinated acids. The method is robust (i.e., capable of measuring neutral and acidic compounds), and can be applied for the analysis of a range of poly- and perfluorinated acids, including telomer alcohols, telomer acids, perfluoroalkylcarboxylates, and perfluoroalkylsulfonates in water and biota. With the use of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), a method detection limit in the range of several tens to hundreds of parts-per-quadrillion (pg/L) in water and at a few tens to hundreds of parts-per-trillion (pg/g) levels in biological matrices can be achieved. (c) 2005 Elsevier B.V. All rights reserved.