A graphene-based platform for fluorescent detection of SNPs.
A graphene-based platform for fluorescent detection of SNPs.
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DOI：
10.1039/c3an36740a
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发表时间：
2013-04
期刊：
影响因子：
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通讯作者：
Hui Xu;Qing Yang;Fan Li;Linsheng Tang;Shanmin Gao;Bowei Jiang;Xingchun Zhao;Lihua Wang;C. Fan
中科院分区：
文献类型：
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作者：
Hui Xu;Qing Yang;Fan Li;Linsheng Tang;Shanmin Gao;Bowei Jiang;Xingchun Zhao;Lihua Wang;C. Fan
利用氧化石墨烯（GO）建立了一种新的荧光单核苷酸多态性（SNP）检测方法，为SNP检测提供了一种快速、灵敏、简便的方法。该策略基于单碱基延伸反应和荧光素标记的dGTP（dGTP-Fl）和双链DNA（dsDNA）对GO的不同吸收能力。dGTP-Fl通过突变体靶的延伸反应而不是野生体靶的延伸反应掺入探针中，这导致突变体靶的荧光恢复，因为dsDNA和GO之间的弱相互作用，而野生体靶的荧光弱，因为dGTP-Fl的荧光被GO淬灭。该方法显示了从3 nM至50 nM的μ-型靶标的线性范围，3 nM为检测限。注意到，在野生型靶标存在下可以检测到低至10%的muplant型靶标，其中浓度是muplant型靶标的9倍。
A novel fluorescent single nucleotide polymorphism (SNP) assay was developed by using Graphene Oxide (GO), which provides a fast, sensitive and simple method for SNP detection. The strategy was based on the single base extension reaction and different absorption capacity of fluorescein labeled dGTP (dGTP-Fl) and double-stranded DNA (dsDNA) to GO. dGTP-Fl is incorporated into the probe by extension reaction for the mutant target but not for the wild target, which leads to recovered fluorescence for the mutant target because of weak interaction between dsDNA and GO and weak fluorescence for the wild target because of the quenched fluorescence of dGTP-Fl by GO. The method shows a linear range for the mutant-type target from 3 nM to 50 nM and 3 nM is the detection limit. It was noted that as low as 10% mutant-type target could be detected in the presence of the wild-type target, in which the concentration is 9 times higher than that of the mutant-type target.