Sub3 inhibits Aspergillus flavus growth by disrupting mitochondrial energy metabolism, and has potential biocontrol during peanut storage.
Sub3 inhibits Aspergillus flavus growth by disrupting mitochondrial energy metabolism, and has potential biocontrol during peanut storage.
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DOI：
10.1002/jsfa.10657
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发表时间：
2020-07
影响因子：
4.1
通讯作者：
Wei Zhang;Yangyong Lv;Ang Lv;Sha Wei;Shuaibing Zhang;Cuixiang Li;Yuan-sen Hu
中科院分区：
文献类型：
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作者：
Wei Zhang;Yangyong Lv;Ang Lv;Sha Wei;Shuaibing Zhang;Cuixiang Li;Yuan-sen Hu
背景黄曲霉是一种腐殖真菌，经常在含油种子中检测到。在定殖过程中，这种微生物释放黄曲霉毒素，对食品安全和人类健康构成严重威胁。因此，需要一种生态友好的生物方法来抑制病原体。结果A.当Sub 3浓度超过0.15 g L-1时，黄曲霉孢子在马铃薯葡萄糖肉汤培养基中不能萌发。通过流式细胞术和扫描电子显微镜进行的形态学评价表明，孢子在Sub 3暴露后收缩并有凹坑。使用碘化丙啶、5，5 '，6，6'-四氯-1，1 '，3，3'-四乙基苯并咪唑羰花青碘化物、2，7-二氯二氢荧光素二乙酸酯和4 '，6-二脒基-2-苯基吲哚染色进行的生理学评估显示细胞膜受损、线粒体膜电位降低、细胞内活性氧水平升高以及大核凝聚和DNA片段化升高。0.1和0.15 g L ~（-1）Sub 3处理后，线粒体脱氢酶活性分别降低了29.42%和45.48%。此外，Sub 3还显著降低了花生种子的定殖能力，与对照组相比，Sub 3处理的种子上孢子数分别减少了26.86%（0.1gL-1）和77.74%（0.15gL-1）。结论Sub 3可能抑制A.通过穿过细胞壁并靶向细胞膜，破坏线粒体能量代谢，并诱导DNA损伤，导致孢子死亡，从而破坏黄孢霉。因此，Sub 3可能是一种有效的生物防治策略。花生的黄变生长本文受版权保护。All rights reserved.
BACKGROUND Aspergillus flavus, a saprophytic fungus, is regularly detected in oil-enriched seeds. During colonisation, this organism releases aflatoxins that pose a serious risk to food safety and human health. Therefore, an eco-friendly biological approach to inhibit the pathogen is desirable. RESULTS Experimental results indicated that A. flavus spores could not germinate in potato dextrose broth culture medium, when the concentration of Sub3 exceeded 0.15 g L-1 . Morphological evaluation performed by flow cytometry and scanning electron microscopy indicated that spores were shrunken and pitted following Sub3 exposure. Physiological assessment using propidium iodide, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide, 2,7-dichlorodihydrofluorescein diacetate and 4',6-diamidino-2-phenylindole staining revealed damaged cell membranes, decreased mitochondrial membrane potential, increased intracellular reactive oxygen species levels, and elevated large nuclear condensation and DNA fragmentation. Moreover, mitochondrial dehydrogenase activity was reduced by 29.42% and 45.48% after treatment with 0.1 and 0.15 g L-1 Sub3, respectively. Additionally, colonization capacity in peanut was significantly decreased, and the number of spores on seeds treated with Sub3 was decreased by 26.86% (0.1 g L-1 ) and 77.74% (0.15 g L-1 ) compared with the control group. CONCLUSION Sub3 likely inhibits A. flavus by crossing the cell wall and targeting the cell membrane, disrupting mitochondrial energy metabolism, and inducing DNA damage, leading to spore death. Thus, Sub3 may provide a useful biocontrol strategy to control A. flavus growth in peanuts. This article is protected by copyright. All rights reserved.