STRUCTURE OF A TRANSCRIPTIONAL UNIT ON COLICINE-1 PLASMID
STRUCTURE OF A TRANSCRIPTIONAL UNIT ON COLICINE-1 PLASMID
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DOI：
10.1111/j.1432-1033.1979.tb13131.x
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
OKA, A
中科院分区：
文献类型：
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作者：
MORITA, M;OKA, A
在体外RNA合成系统中，在大肠杆菌素E1质粒（ColE 1）及其缺失衍生物（来自大肠杆菌分子）的DNA上有效合成了由约110个残基组成的低分子量RNA（RNA-I）。通过测试RNA聚合酶结合能力和限制性片段的模板活性来分析RNA-I的启动子位点;它被定位在ColE 1的复制起始位点和大肠杆菌素免疫基因之间的区域。通过对模板的分离链进行变性测试来确定转录方向。对RNA-I的DNA序列进行了测定，并根据最近邻序列对RNA-I进行了归属。这种小RNA的功能尚不清楚，根据其序列和对RNase的敏感性可以构建出独特的二级结构。
In a RNA-synthesizing system in vitro, a low-MW RNA consisting of about 110 residues (RNA-I) was efficiently synthesized on DNA of colicin E1 plasmid (ColE1) and its deletion derivatives [from Escherichia coli molecules]. The promoter site for RNA-I was analyzed by testing the RNA polymerase-binding ability and template activity of restriction fragments; it was mapped in the region between the replication initiation site and the colicin immunity gene of ColE1. The direction of transcription was determined by hydridization tests to the separated strands of the template. The DNA region directing RNA-I was sequenced, and RNA-I was assigned on the sequence based on the nearest-neighbor the function of this small RNA species is unknown, a unique secondary structure could be constructed from its sequence and sensitivity to RNase.