HISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON HELA CELL CULTURES EXPOSED TO SPINDLE INHIBITORS WITH SPECIAL REFERENCE TO THE INTERPHASE CELL
HISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON HELA CELL CULTURES EXPOSED TO SPINDLE INHIBITORS WITH SPECIAL REFERENCE TO THE INTERPHASE CELL
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对暴露于纺锤体抑制剂的 HELA 细胞培养物的组织化学和超微结构研究，特别是间期细胞
DOI：
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发表时间：
1964
影响因子：
3.2
通讯作者：
N. Gonatas
中科院分区：
文献类型：
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作者：
E. Robbins;N. Gonatas
HeLa(S3)株的哺乳动物细胞在接触纺锤体抑制剂时，在间期和有丝分裂期间都经历了几次形态变化。其中一些已经进行了组织化学和超微结构的研究。在HeLa细胞中，以多泡小体为代表的溶酶体形成簇状排列，而不是占据这些细胞器所特有的极化的核旁位置。在电子显微镜下可以看到，它们已经获得了一个致密的嗜奥米核，该核心与结合单元膜被一个电子发光晕分开。高尔基体在纺锤体抑制剂的影响下破碎，也以类似于溶酶体周向分布的方式出现。在超微结构水平上，该细胞器未见明显变化。间期细胞中还有许多60-80ä的纤维穿过细胞质，以及少许纺锤体，我们的微管。在药物处理的间期细胞和未处理的正常有丝分裂细胞中，溶酶体的行为有惊人的相似性。从微管消失引起的原生质流中断的角度，对所提到的一些变化给出了可能的解释。
Mammalian cells of the HeLa (S3) strain, when exposed to spindle inhibitors, have been found to undergo several morphological transformations during interphase as well as during mitosis. Some of these have been studied both histochemically and ultrastructurally. The lysosomes, represented by the multivesicular bodies in HeLa cells, form clusters and become circumferentially disposed instead of occupying the polarized juxtanuclear position characteristic of these organelles. In the electron microscope it is seen that they have acquired a dense osmiophilic core that is separated from the bounding unit membrane by an electron lucent halo. The Golgi apparatus fragments under the influence of spindle inhibitors and also takes up a circumferential distribution in a pattern similar to that of the lysosomes. On the ultrastructural level, no significant modifications in this organelle are seen. Also noted in the interphase cell are numerous 60-80 Å fibrils coursing through the cytoplasm as well as a paucity of spindle our microtubules. A striking similarity has been pointed out between the behavior of the lysosomes in the drugtreated interphase cell and the untreated, normal mitotic cell. A possible explanation of some of the changes noted has been given in terms of an interruption of protoplasmic flow resulting from the disappearance of microtubules.