Object familiarization and novel-object preference in rats
Object familiarization and novel-object preference in rats
复制标题
DOI：
10.1016/j.beproc.2009.10.003
复制
发表时间：
2010-01-01
影响因子：
1.3
通讯作者：
Mumby, Dave G.
中科院分区：
文献类型：
--
作者：
Gaskin, Stephane;Tardif, Marilyn;Mumby, Dave G.
在大鼠的新奇物体偏好(NOP)测试中，我们研究了物体熟悉程度与新奇物体偏好之间的关系。在实验1中，我们发现，研究一个样本对象的两个相同副本所花费的时间与对一个新对象的偏好程度之间没有显著的相关性。在实验2中，大鼠调查了两个相同的样本对象，总共持续了5、30、60、90或120s。对新对象的研究偏好与机会预期进行了比较，并在两组之间进行了比较。只有90-S组和120-S组表现出对新奇对象的概率调查偏好，但这两组的新奇对象偏好没有差异，这表明大鼠需要进行最少量的样本物调查才能形成新奇对象偏好，超过这一点，大鼠的新奇对象偏好没有增加。在实验3和实验4中，对正常大鼠和海马区损伤大鼠进行重复测试，同一样本对象呈现不同的新对象，在24小时、(实验3)和35-S间隔(实验4)。在这两个实验中，对新对象的偏好并没有随着重复暴露样本对象的增加而增加，这表明对样本对象的熟悉程度的增加并不会导致对新对象偏好的增加。背侧海马区受损的大鼠对这一新对象表现出不可靠的研究偏好。根据NOP测试作为评估大鼠物体识别记忆的工具的潜在局限性，对这些结果进行了讨论。(C)2009爱思唯尔B.V.保留所有权利。
We investigated whether object familiarization was related to novel-object preference in the novel-object preference (NOP) test in rats. In Experiment 1, we found that no significant correlation existed between the time spent investigating 2 identical copies of a sample object and the degree of preference for a novel object. In Experiment 2, rats investigated 2 identical sample objects for a total of 5, 30, 60, 90 or 120s. Investigatory preference for the novel object was compared to chance expectancy as well as between the groups. Only the 90-s group and the 120-s group displayed above-chance investigatory preference for the novel object, but novel-object preference for these 2 groups did not differ from each other, suggesting that a minimal amount of sample object investigation is necessary for rats to develop a novel-object preference, beyond which no increase in novel-object preference was found. In Experiments 3 and 4, normal rats and rats with hippocampal lesions were given repeated test trials, with the same sample object presented with a different novel object, at 24-h and (Experiment 3) and 35-s intervals (Experiment 4). In both experiments, novel-object preference did not increase in magnitude with repeated sample object exposures, suggesting that increased familiarity with the sample object does not result in increased novel-object preference. Rats with lesions of the dorsal hippocampus showed an unreliable investigatory preference for the novel object. These results are discussed in terms of the potential limitations of the NOP test as a tool for the assessment of object-recognition memory in rats. (C) 2009 Elsevier B.V. All rights reserved.