Coordinate regulation of the IL-4, IL-13, and IL-5 cytokine cluster in Th2 clones revealed by allelic expression patterns
Coordinate regulation of the IL-4, IL-13, and IL-5 cytokine cluster in Th2 clones revealed by allelic expression patterns
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DOI：
10.4049/jimmunol.165.6.2982
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发表时间：
2000-09-15
影响因子：
4.4
通讯作者：
Locksley, RM
中科院分区：
文献类型：
--
作者：
Kelly, BL;Locksley, RM
细胞因子 IL-4、IL-13 和 IL-5 是效应 T 细胞 Th2 亚群的标志物，通常一起表达。在小鼠和人类中，这些细胞因子基因均组织在 140 kb 的直系同源 DNA 内。使用源自F-1小鼠的表达IL-1的CD4(+)T细胞克隆，我们鉴定了每种细胞因子的等位基因多态性，并评估了细胞因子mRNA的亲本身份。每个基因以及另外的基因IL-3均发生单等位基因和双等位基因表达，IL-3与GM-CSF位于同一染色体上超过450 kh端粒。当在 T 细胞克隆中共表达时，81% 的情况下，IL-4 与 IL-13 或 IL-5 表达自相同的等位基因。相比之下，表达IL-3的克隆中这三种细胞因子在等位基因水平上的一致性只有52%，细胞因子等位基因的独立表达通常发生在T细胞中，但包含IL-4、IL-13和IL-5的聚集位点受到协调调控。
The cytokines IL-4, IL-13, and IL-5 are markers for the Th2 subset of effector T cells and are often expressed together. These cytokine genes are organized within 140 kb of orthologous DNA in both mouse and human. Using IL-1-expressing CD4(+) T cell clones derived from F-1 mice, we identified allelic polymorphisms for each of these cytokines and assessed the parental identity of the cytokine mRNAs, Both monoallelic and biallelic expression occurred for each gene and for an additional gene, IL-3, that lies with GM-CSF over 450 kh telomeric on the same chromosome. When coexpressed in T cell clones, IL-4 was expressed from the same allele as IL-13 or IL-5 in 81% of instances. In contrast, there was only 52% concordance of these three cytokines at the allelic level among clones that expressed IL-3, Independent expression of the cytokine alleles occurs commonly in T cells, but the clustered locus encompassing IL-4, IL-13, and IL-5 is subject to coordinate regulation.