Electroacupuncture Improves Insulin Resistance by Reducing Neuroprotein Y/Agouti-Related Protein Levels and Inhibiting Expression of Protein Tyrosine Phosphatase 1B in Diet-induced Obese Rats
Electroacupuncture Improves Insulin Resistance by Reducing Neuroprotein Y/Agouti-Related Protein Levels and Inhibiting Expression of Protein Tyrosine Phosphatase 1B in Diet-induced Obese Rats
复制标题
电针通过降低饮食诱导肥胖大鼠神经蛋白 Y/Agouti 相关蛋白水平并抑制蛋白酪氨酸磷酸酶 1B 表达改善胰岛素抵抗
DOI：
10.1016/j.jams.2015.11.037
复制
发表时间：
2016-04-01
影响因子：
1
通讯作者：
Jiang, Peng-Fei
中科院分区：
文献类型：
--
作者：
Liu, Xia;He, Jun-Feng;Jiang, Peng-Fei
电针(EA)已被证明对肥胖有有益效果，但其机制尚不清楚。本研究观察电针对饮食诱导肥胖(DIO)大鼠的影响。雄性SD大鼠50只，随机分为低脂饮食组(LFD，10只)和高脂饮食组(HFD，40只)。建立DIO模型后，将造模成功的大鼠随机分为HFD组、电针组和奥利司他(OLST)组。电针组针刺足三里、曲池穴，每日1次，每次20分钟，疗程28天。OLST组给予奥利司他灌胃治疗。电针组体重、稳态模型评估胰岛素抵抗指数、脂肪细胞直径、神经蛋白Y/刺鼠相关蛋白和蛋白酪氨酸磷酸酶1B水平显著低于高脂组。OLST组大鼠大便呈水样，毛发呈黄色，而电针组大鼠大便规则，皮毛光滑。电针的减肥作用可能与改变脂肪细胞大小、减少神经蛋白Y/刺鼠相关蛋白和蛋白酪氨酸磷酸酶1B的表达而改善胰岛素抵抗有关。这项研究表明，电针可能是治疗肥胖和其他代谢性疾病的一种更好的替代疗法。
Electroacupuncture (EA) has been shown to exert beneficial effects on obesity, but the mechanism is unclear. This study investigated the effects of EA on diet-induced obese (DIO) rats. Fifty male Sprague-Dawley rats were randomly divided into low-fat diet (LFD, 10 rats) and high-fat diet (HFD, 40 rats) groups. After the DIO models had been established, successful model rats were randomly divided into HFD, EA, and orlistat (OLST) groups. The EA group received EA at Zusanli (ST36) and Quchi (LI11) for 20 minutes once per day for 28 days. The OLST group was treated with orlistat by gavage. The body weight, homeostasis model assessment-insulin resistance index, adipocyte diameters, and neuroprotein Y/agouti-related protein and protein tyrosine phosphatase 1B levels were significantly lower in the EA group than in the HFD group. The rats of the OLST group showed watery stools and yellow hairs whereas those of the EA group had regular stools and sleek coats. The effect of EA on weight loss may be related to improved insulin resistance caused by changes in the adipocyte size and by reductions in the expressions of neuroprotein Y/agouti-related protein and protein tyrosine phosphatase 1B. This study indicates that EA may be a better method of alternative therapy for treating obesity and other metabolic diseases.