Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification
Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification
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DOI：
10.1038/sj.onc.1206021
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发表时间：
2002-11-21
期刊：
影响因子：
8
通讯作者：
Lee, JJ
中科院分区：
文献类型：
--
作者：
El-Naggar, AK;Kim, HW;Lee, JJ
与头颈部鳞状癌（HNSC）的发生和进展相关的遗传事件在很大程度上是未知的。我们分析了12对组织学正常的鳞状黏膜和肿瘤标本，分别来自6个常规和6个表型变异的HNSC，以确定这些肿瘤中的差异表达基因。对8055个独特基因进行平行表达分析，归一化后测定每个基因的杂交信号水平。表达基因的层次聚类分析显示，在常规鳞状癌和鳞状癌变体中，肿瘤间和肿瘤内的模式不同。我们还发现了26个（0.32%）差异表达基因，这些基因在配对对正常和肿瘤标本之间始终存在差异；选择一组过表达基因使用实时定量RT-PCR进行验证。大多数基因与分化和增殖有关。我们的研究定义了一组基因，可以为构建有限的HNSC靶向表达阵列带深度研究奠定基础，并进一步突出基因谱差异，可能有助于HNSC的病理分类。
The genetic events associated with the development and progression of head and neck squamous carcinoma (HNSC) are largely unknown. We analysed 12 matched pairs of histologically normal squamous mucosa and tumor specimens from six conventional and six phenotypic variants HNSC to define the differentially expressed genes in these tumors. Parallel expression analysis of 8055 unique genes was performed, and the level of the hybridization signal for each gene was measured after normalization. Hierarchical cluster analysis of the expressed genes showed distinct inter-and intra-tumoral patterns in and between conventional squamous carcinoma and squamous carcinoma variants. We also identified 26 (0.32%) differentially expressed genes that were consistently different between matched pairs of normal and tumor specimens; a selected set of the overexpressed genes was validated using real-time quantitative RT-PCR. The majority of the genes were associated with differentiation and proliferation. Our study defines a set of genes that could form the basis for the construction of limited HNSC targeted expression array band indepth studies and further highlights gene profile differences that may be useful in pathobiologic classification of HNSC.