Prevalence of Porphyromonas gingivalis in relation to periodontal status assessed by real-time PCR
Prevalence of Porphyromonas gingivalis in relation to periodontal status assessed by real-time PCR
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DOI：
10.1111/j.1399-302x.2004.00154.x
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发表时间：
2004-10-01
影响因子：
--
通讯作者：
Koga, T
中科院分区：
文献类型：
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作者：
Kawada, M;Yoshida, A;Koga, T
许多研究已经检查了牙龈卟啉单胞菌在牙周袋中的存在。然而，监测细菌细胞的数量是困难的。在这项研究中，我们对牙龈卟啉单胞菌进行了定量分析，以阐明这种微生物的数量与牙周状况之间的关系。使用TaqMan实时PCR系统，我们发现牙龈卟啉单胞菌的数量与牙周袋深度之间存在显著的正相关性（P < 0.0001）。回归线的斜率表明，牙周袋深度每增加1 mm，牙龈卟啉单胞菌的数量增加10倍。治疗前后牙龈卟啉单胞菌数量也明显减少（P < 0.01）。这些结果表明，牙龈卟啉单胞菌的绝对和相对数量与牙周状况密切相关，这种生物体的定量分析是重要的牙周治疗的评价。
Many studies have examined the presence of Porphyromonas gingivalis in periodontal pockets. However, monitoring the number of bacterial cells is difficult. In this study, we performed quantitative analyses of P. gingivalis to clarify the relationship between the numbers of this organism and periodontal status. Using the TaqMan real-time PCR system, we found a significant positive correlation (P < 0.0001) between the number of P. gingivalis and pocket depth. The slope of the regression line indicated that for every 1-mm increase in pocket depth, the number of P. gingivalis increased 10- fold. There was also a significant reduction (P < 0.01) in the numbers of P. gingivalis before and after treatment. These results suggest that the absolute and relative numbers of P. gingivalis are closely associated with periodontal status, and that quantitative analysis of this organism is important for the evaluation of periodontal therapy.