Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages
Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages
复制标题
桑黄多糖通过调节 LPS 刺激的 RAW264.7 巨噬细胞中 NF-κ B 易位的抗炎活性
DOI：
10.1016/j.ijbiomac.2019.02.023
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发表时间：
2019-05-15
影响因子：
8.2
通讯作者：
Chen, Linghua
中科院分区：
文献类型：
--
作者：
Xie, Zhenglu;Wang, Yang;Chen, Linghua
炎症反应仍然是疾病最常见和最严重的并发症之一。为深入了解桑黄多糖与炎症的关系，采用脂多糖作用RAW264.7细胞建立炎症细胞模型。结果表明，中药能降低致炎细胞因子(TNF-α、IL-1β、IL-2、IL-6、IL-12)含量及mRNA表达水平，提高抗炎细胞因子(IL-4、IL-10)含量及mRNA表达水平。此外，核因子-kappa B易位水平显著降低，这与I-kappa Bα磷酸化水平降低和AMPKα磷酸化水平升高有关。提示中药能减轻内毒素诱导的炎症细胞模型的炎症反应，可能与其抑制核因子-kappaB转位，调节抗炎与促炎因子的平衡有关。(C)2019爱思唯尔B.V.保留所有权利。
Inflammatory response remains one of the most common and serious complications of disease. In order to profound understanding relationship between Phellinus linteus polysaccharides (TCM) and inflammation, inflammatory cell model was constructed by LPS acting RAW264.7 cell line. The results showed that TCM could decrease the pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-2, IL-6 and IL-12) contents and the mRNA expression levels and increase the anti-inflammatory cytokines (IL-4 and IL-10) contents and the mRNA expression levels. Additionally, the levels of NF-kappa B translocation was significantly decreased, which associated with the I kappa B alpha phosphorylation level decreased and the AMPK alpha phosphorylation level increased. These results indicated that TCM could reduce the inflammatory responses in the LPS induced inflammatory cell model might be related to inhibit NF-kappa B translocation and regulate the balance of anti-inflammatory and pro-inflammatory factors. (C) 2019 Elsevier B.V. All rights reserved.