The effect of complete or specific partial acetimidylation on the biological properties of cytochrome c and cytochrome c-T.
The effect of complete or specific partial acetimidylation on the biological properties of cytochrome c and cytochrome c-T.
复制标题
完全或特定部分乙酰亚胺化对细胞色素 c 和细胞色素 c-T 生物学特性的影响。
DOI：
10.1042/bj2170595
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
C. Wallace
中科院分区：
文献类型：
--
作者：
C. Wallace
马细胞色素c的所有19个ε -氨基乙酰化的生物学后果是，在细胞色素c耗尽的线粒体测定中，蛋白质的氧化还原电位及其刺激氧气摄取的能力略有下降。对其他物种的一些特定的部分乙酰化类似物和乙酰化细胞色素c的研究表明，通过核磁共振光谱监测，生物特性的变化与轻微的结构变化有关[Boswell， Moore, Williams, Harris, Wallace, Bocieck & Welti (1983) Biochem]。[j] . 213, 679-686]，似乎源于序列中有限区域的残基修饰。酿酒酵母细胞色素c的氧化还原电位不受乙酰酰化的影响，这表明该物种中不存在的赖氨酸-53是敏感残基。
The biological consequences of acetimidylation of all 19 epsilon-amino groups of horse cytochrome c are a slight decrease in both the redox potential of the protein and its ability to stimulate oxygen uptake in the cytochrome c-depleted-mitochondria assay. Examination of a number of specific partially acetimidylated analogues and acetimidylated cytochromes c of other species has shown that the changes in biological properties, which are associated with a slight structural change as monitored by n.m.r. spectroscopy [Boswell, Moore, Williams, Harris, Wallace, Bocieck & Welti (1983) Biochem. J. 213, 679-686], appear to stem from modification of residues in a restricted region of the sequence. The failure of the redox potential of Saccharomyces cerevisae cytochrome c to be affected by acetimidylation suggests that it is lysine-53, absent from that species, that is the sensitive residue.