MYOINOSITOL METABOLISM IN NEONATAL AND DEVELOPING RAT FED A MYOINOSITOL-FREE DIET
MYOINOSITOL METABOLISM IN NEONATAL AND DEVELOPING RAT FED A MYOINOSITOL-FREE DIET
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DOI：
10.1093/jn/106.11.1610
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发表时间：
1976-01-01
影响因子：
4.2
通讯作者：
WELLS, WW
中科院分区：
文献类型：
--
作者：
BURTON, LE;RAY, RE;WELLS, WW
Holtzman品系的新生大鼠，6日龄，通过胃插管喂食限制肌醇的液体配方10天，之后喂食纯化的不含肌醇的饮食，直到它们72日龄。在肌醇剥夺的大鼠和喂食补充有114 mg肌醇/100 ml配方或250 mg肌醇/100 g饮食的相同饮食的大鼠之间没有观察到体重增加的差异。除了大脑和小脑外，从喂食肌醇剥夺配方和饮食的大鼠中检查的大多数组织的游离肌醇水平低于对照组。在游离肌醇水平降低的组织中，除肝脏外，脂质结合肌醇正常。尽管肝脏中游离和脂质结合的肌醇减少，但在任何年龄的年轻大鼠中均没有脂肪肝的证据。剥夺肌肌醇的大鼠的大脑和小脑具有正常的髓鞘形成和髓鞘形成，这分别通过2“，3”-环核苷酸-3“-磷酸水解酶（EC 3.1.4.1）和磷酸酶（EC 4.2.1.2）活性的水平来判断。
Neonatal rats of the Holtzman strain, 6 days of age, were fed a myo-inositol restricted liquid formula by gastric intubation for 10 days, after which they were fed a purified myo-inositol-free diet until they were 72 days old. No differences in weight gain were observed between myo-inositol deprived rats and those fed the same diet supplemented with 114 mg myo-inositol/100 ml of formula or 250 mg myo-inositol/100 g diet. Most tissues examined from rats fed the myo-inositol deprived formula and diet had lower free myo-inositol levels than the controls with the exception of the cerebrum and cerebellum. Among those tissues having reduced free myo-inositol levels, the lipid-bound myo-inositol was normal with the exception of the liver. Despite reduced free and lipid-bound myo-inositol in the liver, there was no evidence of fatty liver in the young rats at any age. The cerbrum and cerebellum of myo-inositiol deprived rats had normal myelination and mitochondriogenesis as judged by the levels of 2'',3''-cyclic nucleotide-3''-phosphohydrolase (EC 3.1.4.1) and fumarase (EC 4.2.1.2) activity, respectively.