Identification and functional analysis of ABC transporter genes related to deltamethrin resistance in Culex pipiens pallens
Identification and functional analysis of ABC transporter genes related to deltamethrin resistance in Culex pipiens pallens
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DOI：
10.1002/ps.7539
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发表时间：
2023-05-26
影响因子：
4.1
通讯作者：
Shen，Bo
中科院分区：
文献类型：
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作者：
Xu，Jingwei;Zheng，Junnan;Shen，Bo
背景：在蚊子体内繁殖或发展的病原体可以传播多种疾病，危害人类健康，并使卫生系统不堪重负。合成拟除虫菊酯是最广泛使用的杀虫剂，但它们的广泛使用已导致抗药性。三磷酸腺苷结合盒(ABC)转运蛋白参与昆虫抗药性监测，但其在昆虫抗药性中的作用和机制尚未完全阐明。在本研究中，我们鉴定了淡色库蚊的ABC转运蛋白基因，并探讨了它们在抗药性形成中的作用。并根据ABC转运蛋白亚家族对其进行分类。我们还进行了系统发育分析。与对照组相比，口服ABC转运蛋白抑制剂维拉帕米的蚊子在溴氰菊酯处理后的击倒率增加。ABCB、ABCC和ABCG亚家族的几个基因在抗药性蚊子中高度表达。免疫荧光分析表明，ABCG6032427在头部、胸部、腹部、翅膀和腿部表达，以腿部表达最高。随后，利用RNA干扰技术(RNAi)敲除ABCG6032427基因，提高了蚊虫对溴氰菊酯的敏感性，扫描和透射电子显微镜显示，ABCG6032427基因敲除后，蚊体对溴氰菊酯的抗性降低，角质层变得疏松和不规则。结论ABCG6032427基因可能调节了蚊体的角质层厚度和结构，在蚊虫对溴氰菊酯的抗性形成过程中发挥了重要作用。因此，它可能成为Cx对溴氰菊酯抗性管理的一个潜在目标。皮皮恩斯。©2023化学工业学会。
BACKGROUNDPathogens that reproduce or develop in mosquitoes can transmit several diseases, endanger human health, and overwhelm health systems. Synthetic pyrethroids are the most widely used insecticides against adult mosquitoes, but their widespread use has led to resistance. The adenosine triphosphate (ATP)‐binding cassette (ABC) transporters are involved in the resistance monitoring of insects, but their role and underlying mechanisms in insecticide resistance have not been fully elucidated. In the present study, we identified ABC transporter genes inCulex pipiensand investigated their role in the development of insecticide resistance.RESULTSWe identified 63 ABC transporter genes inCx. pipiensand classified them as per the ABC transporter subfamilies. We also performed phylogenetic analysis. The knockdown rate of the mosquitoes orally fed with the ABC transporter inhibitor verapamil increased after deltamethrin treatment compared with that of the control group. Several genes from the ABCB, ABCC, and ABCG subfamilies were highly expressed in resistant mosquitoes. Immunofluorescence analysis revealed thatABCG6032427was expressed in the head, chest, abdomen, wings, and legs, and the expression was the highest in the legs. Subsequently, knockdown ofABCG6032427using RNA interference (RNAi) increased the sensitivity of the mosquitoes to deltamethrin, and scanning and transmission electron microscopy revealed thatABCG6032427knockdown reduced cuticle thickness and the cuticle became loose and irregular.CONCLUSIONSABCG6032427may modulate cuticle thickness and structure, thus play an important role in the development of deltamethrin resistance in mosquitoes. Thus, it could be a potential target for deltamethrin resistance management inCx. pipiens. © 2023 Society of Chemical Industry.