Molecular cloning and characterization of ribosomal RNA genes from a blue-green alga, Anacystis nidulans

Molecular cloning and characterization of ribosomal RNA genes from a blue-green alga, Anacystis nidulans
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蓝绿藻 Anacystis nidulans 核糖体 RNA 基因的分子克隆和表征

DOI:
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发表时间:
1981
期刊:
Molecular and General Genetics MGG
影响因子:
--
通讯作者:
M. Sugiura
M. Sugiura
中科院分区:
--
文献类型:
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作者:
N. Tomioka;K. Shinozaki;M. Sugiura

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本研究克隆了两个编码尼杜氏无鱼腥藻rRNA基因的PstI片段(5.3×106和4.3×106道尔顿)。用限制性内切酶图谱、DNA-RNA杂交分析和R-loop技术对克隆的rDNA进行了鉴定。结果表明,这两个片段分别含有16S、23S和5S rRNA基因。在16S和23S rRNA基因之间的间隔区检测到一个tRNA基因(S)。假单胞菌rRNA基因的结构类似于大肠杆菌和裸藻的叶绿体,而不是高等植物的叶绿体。
SummaryTwo PstI fragments (5.3x106 and 4.3x106 daltons) coding for Anacystis nidulans rRNA genes were cloned. The cloned rDNAs were characterized by restriction endonuclease mapping, DNA-RNA hybridization analysis and the R-loop technique. The results indicated that both fragments contained 16S, 23S and 5S rRNA genes in this order. A tRNA gene(s) was detected in the spacer region between 16S and 23S rRNA genes. The organization of A. nidulans rRNA genes resembles those of E. coli and of Euglena chloroplasts rather than those of higher plant chloroplasts.
DOI: --
发表时间: 1980
期刊: The Journal of biological chemistry
影响因子: --
作者:
OrozcoJr,EM;Gray,PW;Hallick,RB
通讯作者: Hallick,RB