Hemopoietic effects of short-term in vivo treatment of mice with various doses of rhG-CSF.
Hemopoietic effects of short-term in vivo treatment of mice with various doses of rhG-CSF.
复制标题
不同剂量rhG-CSF短期体内治疗小鼠的造血作用。
作者：
Z. Pojda;G. Molineux;T. Dexter
正常成年雄性BDF1小鼠皮下注射重组人粒细胞集落刺激因子(rhG-CSF)，每日2次，连续4~5天，剂量10~2500微克/公斤/天。从实验开始每天检测外周血细胞的数量和形态，连续6天。治疗第5天检测脾重量和细胞数、骨髓形态和细胞数、脾和骨髓粒细胞-巨噬细胞集落形成细胞(GM-CFCs)或多潜能干细胞(CFU-S)含量以及肺、肝组织学变化。我们观察到血液中中性粒细胞的数量随剂量的增加而增加，重量、细胞密度以及脾中GM-CFF和CFU-S的数量增加。骨髓细胞密度降至正常的40%。骨髓中GM-CFCs和CFU-S的数量也减少，骨髓形态检查显示红细胞生成受到抑制。
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was injected s.c., twice daily, into normal adult male BDF1 mice for a period of 4-5 days in doses ranging from 10 to 2500 micrograms/kg/day. The number of peripheral blood cells and their morphology was examined daily from the beginning of the experiment for 6 days. On the 5th day of treatment parameters such as spleen weight and cellularity, bone marrow morphology and cellularity, content of granulocyte-macrophage colony-forming cells (GM-CFC) or multipotential stem cells (CFU-S) in spleen and marrow, and also histology of lung and liver were examined. We observed a dose-dependent increase in the number of blood neutrophils, and an increase in weight, cellularity, and numbers of GM-CFC and CFU-S in the spleen. In the bone marrow, cellularity decreased to 40% of normal. Numbers of GM-CFC and CFU-S in marrow were also decreased, and examination of marrow morphology revealed an inhibition of erythropoiesis.