Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications
Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications
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DOI：
10.1021/ja0469333
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发表时间：
2004-10-06
影响因子：
15
通讯作者：
Nagano, T
中科院分区：
文献类型：
--
作者：
Hanaoka, K;Kikuchi, K;Nagano, T
由于可螯合锌在许多生物体系中起着重要作用，因此近年来可螯合锌的检测在生物学研究中受到广泛关注。稀土配合物（Eu 3+，Tb 3+等）对于生物应用具有优异的光谱特性，例如毫秒级的长发光寿命、>200 nm的大斯托克位移和高水溶性。在此，我们提出了一种新的镧系元素传感器分子，[Eu-7]，用于检测Zn 2+的设计和合成。这种铕（Eu 3+）配合物采用喹啉基配体作为发色团和Zn 2+的受体。在向[Eu-7]溶液中加入Zn 2+后，Eu 3+的发光强烈增强，对Zn 2+的选择性高于其他生物学相关的金属阳离子。[Eu-7]的重要优点之一是，与先前报道的Zn 2+敏感发光镧系元素传感器相比，该络合物可以用更长的激发波长（约340 nm）激发，其激发波长对于生物应用而言处于太高的能级。证实了[Eu-7]用于监测活HeLa细胞中Zn 2+变化的有用性。这种新型的Zn 2 +-选择性发光镧系元素化学传感器[Eu-7]应该是一个很好的铅化合物，为开发一系列新型的发光镧系元素化学传感器的生物应用。
Detection of chelatable zinc (Zn2+) in biological studies has attracted much attention recently, because chelatable Zn2+ plays important roles in many biological systems. Lanthanicle complexes (Eu3+, Tb3+, etc.) have excellent spectroscopic properties for biological applications, such as long luminescence lifetimes of the order of milliseconds, a large Stoke's shift of >200 nm, and high water solubility. Herein, we present the design and synthesis of a novel lanthanide sensor molecule, [Eu-7], for detecting Zn2+. This europium (Eu3+) complex employs a quinolyl ligand as both a chromophore and an acceptor for Zn2+. Upon addition of Zn2+ to a solution of [Eu-7], the luminescence of Eu3+ is strongly enhanced, with high selectivity for Zn2+ over other biologically relevant metal cations. One of the important advantages of [Eu-7] is that this complex can be excited with longer excitation wavelengths (around 340 nm) as compared with previously reported Zn2+-sensitive luminescent lamthanide sensors, whose excitation wavelength is at too high an energy level for biological applications. The usefulness of [Eu-7] for monitoring Zn2+ changes in living HeLa cells was confirmed. This novel Zn2+-selective luminescent lanthanide chemosensor [Eu-7] should be an excellent lead compound for the development of a range of novel luminescent lanthanide chemosensors for biological applications.