Physiological effect of chitinase purified from Bacillus subtilis against the tobacco cutworm Spodoptera litura Fab.
Physiological effect of chitinase purified from Bacillus subtilis against the tobacco cutworm Spodoptera litura Fab.
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DOI：
10.1016/j.pestbp.2012.07.002
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发表时间：
2012-09-01
影响因子：
4.7
通讯作者：
Senthil-Nathan, Sengottayan
中科院分区：
文献类型：
--
作者：
Chandrasekaran, Rajamanickam;Revathi, Kannan;Senthil-Nathan, Sengottayan
An extracellular chitinase was purified from Bacillus subtilis. The lethal concentration (LC50) was determined by using chitinase in first, second, and third instars of Spodoptera litura Fab. Chitinase showed the highest insecticidal activity at 6 mu M concentration within 48 h. The nutritional indices were also significantly affected by the 6 mu M concentration (P < 0.05). Food consumption, efficiency of conversion of ingested and digested food, relative growth rate, and consumption values declined significantly while approximate digestibility was increased. Our study indicates that treatment of host plant leaves with the chitinase can regulate (reduce) larval growth and weight, and enhance the mortality. This may serve as an effective biocide and alternative to Bt toxin. (C) 2012 Elsevier Inc. All rights reserved.
