Chemokine (C-X-C Motif) Ligand 12/Stromal Cell-Derived Factor-1 Is Associated With Leukocyte Recruitment in Asthma
Chemokine (C-X-C Motif) Ligand 12/Stromal Cell-Derived Factor-1 Is Associated With Leukocyte Recruitment in Asthma
复制标题
DOI：
10.1378/chest.09-2104
复制
发表时间：
2010-07-10
期刊：
影响因子：
9.6
通讯作者：
Manuel Teran, Luis
中科院分区：
文献类型：
--
作者：
Cristina Negrete-Garcia, Maria;Velazquez, Juan R.;Manuel Teran, Luis
背景：哮喘是一种以广泛白细胞浸润为特征的过敏性气道炎症反应。基质细胞衍生因子（SDF）-1或趋化因子（C-X-C基序）配体12（CXCL 12）吸引许多细胞，包括静息T淋巴细胞、单核细胞、CD 34（+）干细胞、嗜碱性粒细胞和成熟嗜酸性粒细胞。然而，迄今为止，CXCL 12/SDF-1在哮喘白细胞募集中的潜在作用尚未被研究，据我们所知，方法：采用酶联免疫吸附试验测定15例哮喘患者和13例健康人的支气管肺泡灌洗液（BALF）中CXCL 12/SDF-1的水平。结果：哮喘患者BALF中CXCL 12/SDF-1浓度显著高于正常对照组（中位数845 pg/mL，范围296- 1，700 pg/mL vs中位数55 pg/mL，范围25-97 pg/mL; P <0.001）。CXCL 12/SDF-1浓度与巨噬细胞（r = 0.728，P <0.01）、淋巴细胞（r = 0.651，P <0.01）和嗜酸性粒细胞（r = 0.765，P <0.01）相关。结论：CXCL 12/SDF-1在支气管哮喘患者BALF中有高浓度的释放。这种趋化因子的浓度与BALF中白细胞数量相关的发现表明，这种趋化因子可能有助于哮喘中的细胞募集。胸部2010; 138（1）：100-106
Background: Asthma is characterized by allergic airway inflammatory response involving extensive leukocyte infiltration. The stromal cell-derived factor (SDF)-1 or chemolcine (C-X-C motif) ligand 12 (CXCL12) attracts a number of cells, including resting T lymphocytes, monocytes, CD34(+) stem cells, basophils, and mature eosinophils. To date, however, the potential role of CXCL12/SDF-1 in relation to leukocyte recruitment in asthma has not been previously examined, to our knowledge.Methods: Levels of CXCL12/SDF-1 in the BAL fluid (BALF) of 15 subjects with asthma and 13 healthy subjects were measured by enzyme-linked immunosorbent assay. Immunohistochemistry was performed to identify the cellular source of this chemokine.Results: CXCL12/SDF-1 concentrations were significantly elevated in BALF from subjects with asthma compared with normal subjects (median 845 pg/mL, range, 296-1,700 pg/mL vs median 55 pg/mL, range 25-97 pg/mL; P < .001). Concentrations of CXCL12/SDF-1 correlated with macrophages (r = 0.728, P < .01), lymphocytes (r = 0.651, P < .01), and eosinophils (r = 0.765, P < .01). By immunohistochemistry, CXCL12/SDF-1 was localized to the airway epithelium and to a lesser extent to mononuclear cells.Conclusion: CXCL12/SDF-1 is released in high concentration in BALF of patients with asthma. The finding that concentrations of this chemokine correlated with leukocyte numbers in BALF suggests that this chemokine may contribute to the cell recruitment in asthma. CHEST 2010; 138(1):100-106