Compartmentation and topology of glucosylceramide synthesis
Compartmentation and topology of glucosylceramide synthesis
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DOI：
10.1042/bst0280748
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发表时间：
2000-12-01
影响因子：
3.9
通讯作者：
Lynch, DV
中科院分区：
文献类型：
--
作者：
Cantatore, JL;Murphy, SM;Lynch, DV
证据支持葡萄糖神经酰胺在植物质膜质外体侧形成的模型。葡萄糖神经酰胺合酶和甾醇葡萄糖基转移酶都定位于质膜。而甾醇葡萄糖基化是敏感的蛋白水解酶，神经酰胺葡萄糖基化不是。这些结果与我们的模型是一致的，在该模型中，甾醇葡糖苷在膜的胞质侧合成，然后跨膜转运，在那里它将葡萄糖提供给神经酰胺。
Evidence is presented supporting a model for glucosylceramide formation on the apoplastic side of the plasma membrane in plants. Glucosyl-ceramide synthase and sterol glucosyltransferase were both localized to the plasma membrane. Whereas sterol glucosylation was sensitive to proteolytic enzymes, ceramide glucosylation was not. These results are consistent with our model in which steryl glucoside is synthesized on the cytosolic side of the membrane and then translocated across the membrane where it donates glucose to ceramide.