Glycation of β-lactoglobulin and antiglycation by genistein in different reactive carbonyl model systems
Glycation of β-lactoglobulin and antiglycation by genistein in different reactive carbonyl model systems
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DOI：
10.1016/j.foodchem.2015.02.122
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发表时间：
2015-09-15
期刊：
影响因子：
8.8
通讯作者：
Lv, Lishuang
中科院分区：
文献类型：
--
作者：
Kong, Yanghui;Li, Xiaoming;Lv, Lishuang
晚期糖基化终末产物（AGEs）是β-乳球蛋白（β-lg）糖基化系统中通过美拉德反应形成的，与糖尿病相关的长期并发症有关。在本研究中，我们发现反应条件，包括温度，时间，pH值，反应物类型和β-lg与糖/MGO/GO的摩尔比，可以显着影响AGEs的形成。使用SDS-PAGE，我们进一步证明了染料木黄酮，一种在包括大豆和大豆在内的许多植物中发现的天然染料，可以有效地抑制糖化β-Ig的交联，并通过捕获反应性二羰基化合物以剂量依赖性方式抑制AGEs的形成。使用LC/MS分析在β-Ig-MGO测定中由染料木黄酮和甲基乙二醛（MGO）形成的产物。用该方法检测染料木黄酮的单-MGO和二-MGO加合物。(C)2015爱思唯尔有限公司版权所有。
Advanced glycation end products (AGEs), which are formed in B-lactoglobulin (beta-lg) glycation systems via the Maillard reaction, have been implicated in diabetes-related long-term complications. In the present study, we found that reaction conditions, including temperature, time, pH, reactant type and molar ratio of beta-lg to a sugar/MGO/GO, can significantly affect the formation of AGEs. Using SDS-PAGE, we further demonstrated that genistein, a natural isoflavone found in a number of plants including soybeans and kudzu, can efficiently inhibit cross-links of the glycated beta-lg, and suppress the formation AGEs in a dose-dependent manner by trapping reactive dicarbonyl compounds. The products formed from genistein and methylglyoxal (MGO) in the beta-lg-MGO assay were analyzed using LC/MS. Both mono-MGO and di-MGO adducts of genistein were detected with this method. (C) 2015 Elsevier Ltd. All rights reserved.