Nucleolar protein B23 interacts with Japanese encephalitis virus core protein and participates in viral replication
Nucleolar protein B23 interacts with Japanese encephalitis virus core protein and participates in viral replication
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DOI：
10.1111/j.1348-0421.2006.tb03789.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Matsuura, Y
中科院分区：
文献类型：
--
作者：
Tsuda, Y;Mori, Y;Matsuura, Y
日本脑炎病毒（JEV）核心蛋白不仅存在于感染细胞的胞浆中，而且存在于细胞核中。我们以前的研究表明，缺乏核心蛋白核仁定位的突变型JEV会损害哺乳动物细胞中的病毒复制。在这项研究中，我们确定了核仁磷蛋白B23作为一种蛋白结合的核心蛋白的乙脑病毒，但不与登革病毒。与JEV核心蛋白结合的区域定位于B23的氨基酸残基38至77。在JEV感染后，B23的一部分从核仁易位到细胞质中，并且细胞质B23与野生型JEV的核心蛋白共定位，但不与突变型JEV的核心蛋白共定位。此外，B23显性阴性的过表达减少了JEV的复制。这些结果表明，B23在JEV核心蛋白的细胞内定位和复制中起重要作用。
Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphoprotein B23 as a protein binding with the core protein of JEV but not with that of dengue virus. The region binding with JEV core protein was mapped to amino acid residues 38 to 77 of B23. Upon JEV infection, some fraction of B23 was translocated from the nucleoli to the cytoplasm, and cytoplasmic B23 was colocalized with the core protein of wild-type JEV but not with that of the mutant JEV. Furthermore, overexpression of dominant negatives of B23 reduced JEV replication. These results suggest that B23 plays an important role in the intracellular localization of the core protein and replication of JEV.