Accelerated Effect of MatriStem on Dentin Regeneration in Rats
Accelerated Effect of MatriStem on Dentin Regeneration in Rats
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MatriStem 对大鼠牙本质再生的加速作用
DOI：
10.11223/jarde.18.23
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Takashi SAITO
中科院分区：
文献类型：
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作者：
Keiko ONJI;Toshiyuki KOIKE;Mohammad Ali Akbor POLAN;Md Riasat HASAN;Bayarchimeg ALTERHISHIG;Yaxin RAO;Youjin QIU;Yasuhiro MATSUDA;Kazuhisa BESSHO;Takashi SAITO
本研究的目的是观察猪膀胱基质干细胞对活体牙髓修复性牙本质形成和牙髓炎症的影响，并与已用于活髓治疗的三氧化二钙和氢氧化钙进行比较。取8周龄Wistar大鼠上颌骨第一磨牙裸露牙髓组织，用该材料覆盖。术后4周处死大鼠。我们对直接盖髓的牙齿进行了苏木素和伊红染色切片的组织学检查，然后对形成的修复牙本质进行了形态计量分析，并评估了牙髓炎症。4周时，MatriStem形成高度致密、高度修复的牙本质和牙本质小管。与氢氧化钙相比，MatriStem引起的牙髓炎症较少。本研究表明，MatriStem是一种生物相容性、可生物降解性和生物活性的材料，含有骨形态发生蛋白等生长因子用于牙本质再生。
The purpose of the present study was to investigate the effect of MatriStem derived from porcine bladder on reparative dentin formation and pulp inflammation in vivo compared with mineral trioxide aggregate and calcium hydroxide that have been used for vital pulp therapy. Exposed pulp tissue in the maxilla first molar of 8-week-old Wistar rats was covered with those materials. The rats were sacrificed 4 weeks after the operation. We performed histological examination of hematoxylin and eosin-stained sections of teeth that underwent direct pulp capping, followed by morphometric analysis of formed reparative dentin and evaluation of pulp inflammation. At 4 weeks, MatriStem formed highly compact, intensively reparative dentin with dentinal tubules. MatriStem induced less pulp inflammation than calcium hydroxide. This study suggests that MatriStem is a biocompatible, biodegradable, and bioactive material with growth factors such as bone morphogenetic proteins for dentin regeneration.