Fluorogenic atom transfer radical polymerization in aqueous media as a strategy for detection
Fluorogenic atom transfer radical polymerization in aqueous media as a strategy for detection
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DOI：
10.1039/c8sc03938k
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发表时间：
2019-01-28
期刊：
影响因子：
8.4
通讯作者：
Cooley, Christina B.
中科院分区：
文献类型：
--
作者：
Allen, Zachary T.;Sackey-Addo, Jemima R.;Cooley, Christina B.
在水介质中信号放大的新方法的开发可以实现用于检测水溶性分析物（包括生物分子）的新诊断平台。本文描述了一种荧光聚合方法，通过实时检测聚合后的可见荧光来放大引发剂信号。通过原子转移自由基聚合（ATRP）在水中合成并共聚荧光单体，以揭示作为反应时间和引发剂浓度的函数的聚合物荧光增加。荧光ATRP反应条件的优化允许在宽线性浓度范围（pM至mM）内定量检测作为模型分析物的小分子引发剂。将反应温度从30 ° C升高至60 ° C有助于在短至1小时的聚合中在亚皮摩尔浓度下进行灵敏的引发剂检测。然后，该方法被应用于检测链霉亲和素作为一个模型的生物分析物的荧光聚合从设计的生物素化ATRP引发剂。总之，这些研究代表了第一个例子的荧光ATRP反应，并建立荧光聚合作为一个有前途的方法，用于直接检测含水分析物和生物分子识别事件。
The development of novel approaches to signal amplification in aqueous media could enable new diagnostic platforms for the detection of water-soluble analytes, including biomolecules. This paper describes a fluorogenic polymerization approach to amplify initiator signal by the detection of visible fluorescence upon polymerization in real-time. Fluorogenic monomers were synthesized and co-polymerized by atom transfer radical polymerization (ATRP) in water to reveal increasing polymer fluorescence as a function of both reaction time and initiator concentration. Optimization of the fluorogenic ATRP reaction conditions allowed for the quantitative detection of a small-molecule initiator as a model analyte over a broad linear concentration range (pM to mM). Raising the reaction temperature from 30 degrees C to 60 degrees C facilitated sensitive initiator detection at sub-picomolar concentrations in as little as 1 h of polymerization. This method was then applied to the detection of streptavidin as a model biological analyte by fluorogenic polymerization from a designed biotinylated ATRP initiator. Taken together, these studies represent the first example of a fluorogenic ATRP reaction and establish fluorogenic polymerization as a promising approach for the direct detection of aqueous analytes and biomolecular recognition events.