Circulating microparticles in carriers of prothrombin G20210A mutation
Circulating microparticles in carriers of prothrombin G20210A mutation
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DOI：
10.1160/th13-12-1006
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发表时间：
2014-09-01
影响因子：
6.7
通讯作者：
Simioni, Paolo
中科院分区：
文献类型：
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作者：
Campello, Elena;Spiezia, Luca;Simioni, Paolo
凝血因子V Leiden(FVL)和凝血酶原基因突变G20210A(PTM)是已知的两种最常见的基因多态，可使携带者患静脉血栓栓塞症(VTE)。最近在FVL携带者中的一项研究表明，循环中的微粒(MP)水平可能有助于他们的血栓形成特征。为了进一步阐明与遗传性血栓形成有关的血栓前状态，我们将这项研究扩展到PTM的携带者。检测124例PTM携带者(杂合子105例，纯合子19例)和120例年龄、性别匹配的健康人血浆Annexin V-MP、内皮-MP(EMP)、血小板-MP(PMP)、组织因子-MP(Tf+)和MP促凝活性(PPL)水平。杂合子携带者和纯合子携带者的膜联蛋白V-MP水平分别为2930[1440-4646]mP/亩L和3064[2412-4906]mP/亩L，显著低于对照组(分别为1728[782-2122]mP/亩L和71[61-75]秒)；
Factor V Leiden (FVL) and prothrombin gene mutation G20210A (PTM) are the two most common genetic polymorphisms known to predispose carriers to venous thromboembolism (VTE). A recent study in FVL carriers showed that circulating levels of microparticles (MP) may contribute to their thrombogenic profile. To further elucidate the prothrombotic state linked to genetic thrombophilia, we extended this study to carriers of PTM. The plasma level of annexin V-MP, endothelial-MP (EMP), platelet-MP (PMP), tissue factor-bearing MP (TF+) and the MP procoagulant activity (PPL) was measured in 124 carriers of PTM (105 heterozygous and 19 homozygous) and in 120 age- and gender-matched healthy individuals. Heterozygous and homozygous carriers of PTM showed significantly increased levels of annexin V-MP (2930 [1440-4646] MP/mu l and 3064 [2412-4906] MP/mu l, respectively) and significantly shorter PPL clotting time (54 [46-67] sec and 55 [46-64] sec) compared to controls (1728 [782-2122] MP/mu l and 71 [61-75] sec, respectively; p