AtHSPR is involved in GA- and light intensity-mediated control of flowering time and seed set in Arabidopsis.
AtHSPR is involved in GA- and light intensity-mediated control of flowering time and seed set in Arabidopsis.
复制标题
AtHSPR 参与拟南芥中 GA 和光强度介导的开花时间和结籽的控制。
DOI：
10.1093/jxb/eraa128
复制
发表时间：
2020
影响因子：
6.9
通讯作者：
Wang Chongying
中科院分区：
文献类型：
--
作者：
Yang Tao;Sun Yan;Wang Yongli;Zhou Lina;Chen Mengya;Bian Zhiyuan;Lian Yuke;Xuan Lijuan;Yuan Guoqiang;Wang Xinyu;Wang Chongying
开花是一个动态和同步的过程，其时间由各种环境信号进行微调。与野生型相比，拟南芥热休克蛋白相关基因（AtHSPR）的T-DNA插入突变体在长日照（LD）和短日照（SD）条件下均表现出晚开花的表型，而AtHSPR的过表达促进了开花。外源赤霉素（GA）处理部分挽救了LD和SD条件下的晚花突变体表型，表明AtHSPR参与GA生物合成和/或促进开花的GA信号转导。在SD或弱光条件下，athsprmutant表现出开花晚，花粉活力和结实率降低，有缺陷的表型，GA处理部分挽救。qRT-PCR检测结果表明，在低光照和低光照条件下，Athspr-GA生物合成基因表达下调，GA分解代谢基因表达上调，生物活性GA及其中间产物水平降低，进一步表明AtHSPR可能参与了低光照和低光照条件下的GA代谢途径。此外，AtHSPR在体外与GA生物合成中GA 20 ox 1的转录抑制因子OFP 1和KNAT 5相互作用。综上所述，我们的研究结果表明AtHSPR在GA和光照强度介导的开花和结实调节中起着积极的作用。
Flowering is a dynamic and synchronized process, the timing of which is finely tuned by various environmental signals. A T-DNA insertion mutant in ArabidopsisHEAT SHOCK PROTEIN-RELATED(AtHSPR) exhibited late-flowering phenotypes under both long-day (LD) and short-day (SD) conditions compared to the wild-type, while over-expression ofAtHSPRpromoted flowering. Exogenous application of gibberellin (GA) partially rescued the late-flowering mutant phenotype under both LD and SD conditions, suggesting thatAtHSPRis involved in GA biosynthesis and/or the GA signaling that promotes flowering. Under SD or low-light conditions, theAthsprmutant exhibited late flowering together with reduced pollen viability and seed set, defective phenotypes that were partially rescued by GA treatment. qRT-PCR assays confirmed that GA biosynthetic genes were down-regulated, that GA catabolic genes were up-regulated, and that the levels of bioactive GA and its intermediates were decreased inAthsprunder both SD and low-light/LD, further suggesting thatAtHSPRcould be involved in the GA pathway under SD and low-light conditions. Furthermore, AtHSPR interactedin vitrowith OFP1 and KNAT5, which are transcriptional repressors ofGA20ox1in GA biosynthesis. Taken together, our findings demonstrate thatAtHSPRplays a positive role in GA- and light intensity-mediated regulation of flowering and seed set.