Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products.
Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products.
复制标题
DOI：
10.2147/ijn.s50140
复制
发表时间：
2013-01-01
影响因子：
8
通讯作者：
Collins, Andrew M
中科院分区：
文献类型：
--
作者：
Barbour, Michele E;Maddocks, Sarah E;Collins, Andrew M
氯己定（CHX）是一种抗微生物剂，对革兰氏阴性和阳性细菌和酵母菌有效。它的作用机制是基于细胞膜破坏，因此，它不会促进细菌耐药性的发展，这与抗生素的广泛使用有关。在这篇手稿中，我们报告了新的抗菌纳米粒子（NP）的基础上的六偏磷酸盐的CHX的发展。这些是在室温和压力下通过CHX二葡萄糖酸盐和六偏磷酸钠的等摩尔水溶液之间的瞬时反应合成的。反应产生由高度带负电荷的NP（~ 50 mV）组成的稳定胶体，尺寸为20-160 nm。纳米颗粒以剂量依赖性方式快速粘附到玻璃、钛和弹性伤口敷料的样本上。官能化材料在至少50天的时间内表现出可溶性CHX的逐渐浸出。NP胶体对甲氧西林耐药金黄色葡萄球菌（MRSA）和铜绿假单胞菌在渗透性和生物膜条件下均有效。这些纳米颗粒可以在一系列生物医学和消费材料中找到应用。
Chlorhexidine (CHX) is an antimicrobial agent that is efficacious against gram-negative and -positive bacteria and yeasts. Its mechanism of action is based on cell membrane disruption and, as such, it does not promote the development of bacterial resistance, which is associated with the widespread use of antibiotics. In this manuscript, we report the development of novel antimicrobial nanoparticles (NPs) based on a hexametaphosphate salt of CHX. These are synthesized by instantaneous reaction between equimolar aqueous solutions of CHX digluconate and sodium hexametaphosphate, under room temperature and pressure. The reaction results in a stable colloid composed of highly negatively charged NPs (-50 mV), of size 20-160 nm. The NPs adhere rapidly to specimens of glass, titanium, and an elastomeric wound dressing, in a dose-dependent manner. The functionalized materials exhibit a gradual leaching of soluble CHX over a period of at least 50 days. The NP colloid is efficacious against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in both planktonic and biofilm conditions. These NPs may find application in a range of biomedical and consumer materials.