Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering
Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering
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DOI：
10.1163/156856204323046906
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Lee, YM
中科院分区：
文献类型：
--
作者：
Jeong, SI;Kim, SH;Lee, YM
合成了一种柔软、极具弹性的聚丙交酯-己内酯(PLCL)(50：50，M-n185×10(3))。采用挤出-颗粒浸出法制备了用于机械激活血管组织工程的管状支架。该共聚物非常柔软，但完全像橡胶一样有弹性。即使是高孔的PlcL支架(90%盐分重量)也有200%的伸长率，但拉伸测试中的恢复率超过85%。此外，在循环机械应变条件下，PLCL支架在培养液中也保持了其高弹性。高孔度(90%盐分质量分数)支架在10%幅值、1 Hz频率的循环应力作用下，在与自然血管条件相似的条件下，在培养液中维持2周，无任何变形。将血管平滑肌细胞(VSMCs)种植到PLCL支架上。细胞在不同孔径支架上的黏附和增殖均随孔径的增大而增加。50-100微米、100-150微米、150-200微米和200-250微米的细胞数比在12小时和5天时分别约为1：1.2：1.9：2.2。同样，与低孔支架相比，高孔支架表现出更多的细胞黏附和增殖，而低孔支架的细胞黏附和增殖作用在增殖期越长越明显。将SMC种植的支架植入裸鼠皮下，以证实其生物相容性。本研究制备的PLcL支架具有较高的弹性和良好的生物相容性，可用于机械动态环境下的血管等含SM组织工程(机械活性组织工程)。
A soft and very elastic poly(lactide-co-epsilon-caprolactone) (PLCL)(50: 50, M-n 185 x 10(3)) was synthesized. Tubular scaffolds were prepared by an extrusion-particulate leaching method for mechano-active vascular tissue engineering. The copolymer was very flexible but completely rubber-like elastic. Even the high porous PLCL scaffolds (90% salt wt) exhibited 200% elongation, but recovery over 85% in a tensile test. Moreover, the PLCL scaffolds maintained their high elasticity also in culture media under cyclic mechanical strain conditions. The highly porous scaffold (90% salt wt) withstood for an initial 1 week without any deformation and sustained for 2 weeks in culture media under cyclic stress of 10% amplitude and at 1 Hz frequency which are similar to the natural vascular conditions. Vascular smooth muscle cells (VSMCs) were seeded on to the PLCL scaffolds. The cell adhesion and proliferation on the scaffolds of various pore-size were increased with increasing pore size. For the pore sizes of 50-100 mum, 100-150 mum, 150-200 mum and 200-250 mum, the ratios of cell numbers were about 1 : 1.2: 1.9: 2.2, respectively, at both 12 h and 5 days. Similarly, the higher porous scaffolds exhibited more cell adhesion and proliferation compared to lower porous one, where the effect was more pronounced in the longer proliferation period. SMC-seeded scaffolds were implanted subcutaneously in athymic nude mice to confirm the biocompatibility. Such a high elastic property and proper biocompatibility to SMCs of PLCL scaffolds prepared in this study will be very useful to engineer SM-containing tissues such as blood vessels under mechanically dynamic environments (mechano-active tissue engineering).