High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogenesis-related proteins are ribonucleases
High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogenesis-related proteins are ribonucleases
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DOI：
10.1007/bf00201828
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发表时间：
2004
期刊：
影响因子：
4.3
通讯作者：
G. P. Moiseyev;J. Beintema;L. I. Fedoreyeva;G. Yakovlev
中科院分区：
文献类型：
--
作者：
G. P. Moiseyev;J. Beintema;L. I. Fedoreyeva;G. Yakovlev
人参愈伤组织细胞培养的核糖核酸酶。分离到Mey菌株R1。得到了表观分子质量为18 kDa的纯蛋白。测定了该蛋白的n端序列和c端CNBr肽的序列。与其他核糖核酸酶未发现同源性，但与欧芹中2个细胞内致病相关蛋白（IPR）序列有60 ~ 70%的同源性，表明不仅这2个蛋白是核糖核酸酶，在其他植物物种中也发现了同源IPR蛋白。
A ribonuclease from a callus cell culture ofPanax ginsengC.A. Mey strain R1 was isolated. A pure protein with an apparent molecular mass of 18 kDa was obtained. The N-terminal sequences of the protein and of the C-terminal CNBr peptide were determined. No homology with other ribonucleases was found, but there was 60–70% sequence identity with two intracellular pathogenesis-related (IPR) proteins from parsley, indicating that not only these two proteins, but also homologous IPR proteins identified in other plant species are ribonucleases.