Assembly of two types of tubules with putative cytolytic function by cloned natural killer cells
Assembly of two types of tubules with putative cytolytic function by cloned natural killer cells
复制标题
通过克隆的自然杀伤细胞组装两种具有推定溶细胞功能的小管
作者：
E. Podack;G. Dennert
Dourmashkin et al.1和Henkart et al.2最近报告了在人外周血白细胞引起的细胞介导的细胞溶解过程中形成不同大小的超微结构膜损伤。以克隆小鼠自然杀伤（NK）细胞3为效应细胞，YAC-1细胞或兔红细胞为靶细胞，我们报道了内径分别为16±2 nm和105 nm的两种膜损伤。这些损伤是由于在细胞溶解反应期间由亚基组装的管状复合物的膜插入引起的。通过免疫电子显微镜在靶膜上检测到小管，并且似乎形成跨膜通道，如在切片中所见。通过SDS膜提取和含脱氧胆酸盐缓冲液的凝胶过滤对两种小管进行部分纯化。基于微管组装和细胞溶解的相关性及其在靶膜上的检测，我们认为这两种类型的微管可能与细胞溶解有关。
The formation of ultrastructural membrane lesions of varying size during cell mediated cytolysis effected by human peripheral blood leukocytes was recently reported by Dourmashkin et al.1 and Henkart et al.2. Using cloned mouse natural killer (NK) cells3 as effectors and YAC-1 cells or rabbit erythrocytes as targets, we now report two types of membrane lesions with inner diameters of 16±2 nm and ∼5 nm, respectively. These lesions arise by membrane insertion of tubular complexes that may be assembled from subunits during the cytolytic reaction. The tubules are detected on target membranes by immune electron microscopy and appear to form transmembrane channels as seen in ultrathin sections. Both tubules are partially purified by membrane extraction with SDS and gel filtration in deoxycholate containing buffer. Based on the correlation of tubule assembly and cytolysis and on their detection on target membranes, we suggest that both types of tubules may be related to cytolysis.