Switchable adhesion of E. coli to thermosensitive carbohydrate presenting microgel layers: a single cell force spectroscopy study.
Switchable adhesion of E. coli to thermosensitive carbohydrate presenting microgel layers: a single cell force spectroscopy study.
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DOI：
10.1021/acs.langmuir.0c02040
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
Dimitri Wilms;Fabian Schröer;T. Paul;Stephan Schmidt
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文献类型：
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作者：
Dimitri Wilms;Fabian Schröer;T. Paul;Stephan Schmidt
细胞尺度上的黏附过程主要由碳水化合物的相互作用主导，包括病原体的黏附和入侵。碳水化合物递呈的响应性聚合物可以通过远程刺激结合病原体并抑制病原体入侵，从而开发新的抗生素策略。在这项工作中，大肠杆菌与甘露糖功能化微凝胶与热敏性聚N-异丙基丙烯酰胺(PNIPAM)和聚乙二醇寡聚(PEG)网络组成的单层膜的粘附力是用单胞力谱(SCFS)定量的。当超过微凝胶的较低临界溶液温度(LCST)时，根据聚合物主链和甘露糖密度的不同，粘附性最高可增加2.5倍。对于相似的甘露糖密度，较软的PNIPAM微凝胶与较硬的聚乙二醇微凝胶相比，在穿过LCST时表现出显着更强的粘附性增加。这是因为当穿过LCST时，较软凝胶的溶胀度、甘露糖密度和表面粗糙度发生了更大的变化。当使用非结合半乳糖而不是甘露糖，或者当抑制细菌受体时，仍有一定程度的粘附性，这表明聚合物-菌毛缠结也有助于粘附性。该定量分析提供了对碳水化合物介导的细菌黏附及其与材料性能的关系的洞察，并展示了交互聚合物材料在控制细菌黏附方面的前景和局限性。
Adhesion processes on the cellular scale are dominated by carbohydrate interactions, including the attachment and invasion of pathogens. Carbohydrate-presenting responsive polymers can bind pathogens and inhibit pathogen invasion by remote stimulus for the development of new antibiotic strategies. In this work the adhesion forces of E. coli to monolayers composed of mannose-functionalized microgels with thermosensitive poly(N-isopropylacrylamide) (PNIPAM) and poly(oligo(ethylene glycol) (PEG) networks are quantified using single cell force spectroscopy (SCFS). When exceeding the microgels' lower critical solution temperature (LCST), the adhesion increases up to 2.5-fold depending on the polymer backbone and the mannose density. For similar mannose densities the softer PNIPAM microgels show a significantly stronger adhesion increase when crossing the LCST as compared to the stiffer PEG microgels. This is explained by as stronger shift in swelling, mannose density, and surface roughness of the softer gels when crossing the LCST. When using non-binding galactose instead of mannose, or when inhibiting bacterial receptors, a certain level of adhesion remains indicating that also polymer-fimbria entanglements contribute to adhesion. The presented quantitative analysis provides insights into carbohydrate mediated bacteria adhesion, the relation to material properties and shows the prospects but also the limitations of interactive polymer materials to control the attachment of bacteria.