Uterine adenomyosis is associated with ultrastructural features of altered contractility in the inner myometrium
Uterine adenomyosis is associated with ultrastructural features of altered contractility in the inner myometrium
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DOI：
10.1016/j.fertnstert.2009.01.097
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发表时间：
2010-05-01
影响因子：
6.7
通讯作者：
Habiba, Marwan A.
中科院分区：
文献类型：
--
作者：
Mehasseb, Mohamed Khairy;Bell, Stephen Charles;Habiba, Marwan A.
目的：研究子宫腺肌病存在和不存在时子宫内外肌层的超微结构。
设计：病例对照盲法比较。
地点：大学各科室。
患者：4名患有子宫腺肌病且无其他病变的绝经前女性和6名无子宫腺肌病的绝经前女性。
干预措施：使用透射电子显微镜对多个样本进行研究。
主要观察指标：子宫肌层的超微结构特征。
结果：在患有腺肌病的子宫中，肌细胞呈现细胞肥大。细胞质中的肌丝较少。大量中间丝形成细胞质聚集体。细胞核轮廓光滑，基质清晰，核仁明显，核染色质呈周边排列。偶尔可见核膜内折并包裹细胞质细胞器。肌膜带明显更长，小凹更少。核周细胞器更明显。粗面内质网和高尔基体更显著，表明蛋白质合成活跃，这与观察到的细胞肥大一致。所有特征在结合带更为明显。
结论：患有腺肌病的子宫平滑肌细胞在超微结构上与正常子宫的平滑肌细胞不同。这些独特的特征表明，除了肥大之外，可能对子宫肌层的收缩性有影响。（《生育与不育》2010年；93卷：2130 - 2136页。美国生殖医学学会2010年版权所有）
Objective: To study the ultrastructure of the inner and outer myometrium, in the presence and absence of uterine adenomyosis.Design: Case control blinded comparison.Setting: University departments.Patient(s): Four premenopausal women with and six without uterine adenomyosis as the sole pathology.Intervention(s): Multiple samples were studied using transmission electron microscopy.Main Outcome Measure(s): Ultrastructure feature of the myometrium.Result(s): In uteri with adenomyosis, the myocytes exhibited cellular hypertrophy. The cytoplasmic myofilaments were less abundant. Abundant intermediate filaments formed cytoplasmic aggregates. The nuclei had a smooth outline with a clear ground substance, prominent nucleoli and peripherally arranged nuclear chromatin. There was occasional infolding of the nuclear envelope with entrapment of cytoplasmic organelles. The sarcolemmal bands were significantly longer and there were fewer caveolae. The perinuclear cell organelles were more distinct. The rough endoplasmic reticulum and Golgi apparatus were more prominent, denoting active protein synthesis, consistent with the observed cellular hypertrophy. All features were more prominent at the junctional zone.Conclusion(s): Smooth muscle cells from uteri with adenomyosis are ultrastructurally different from smooth muscle cells of normal uteri. These distinct features suggest a possible effect on myometrial contractility, together with hypertrophy. (Fertil Steril (R) 2010;93:2130-6. (C)2010 by American Society for Reproductive Medicine.)