Expression Analysis of Arabidopsis thaliana Small Secreted Protein Genes
Expression Analysis of Arabidopsis thaliana Small Secreted Protein Genes
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DOI：
10.1271/bbb.110649
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发表时间：
2012-03-01
影响因子：
1.6
通讯作者：
Nakagawa, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Nakamura, Shinya;Suzuki, Takamasa;Nakagawa, Tsuyoshi
植物分泌到胞外基质的小分子蛋白调节着许多生理活动，包括病原菌应答、物质运输和形态建成等，但除了一些已知的小分子分泌蛋白外，大多数小分子分泌蛋白的功能还不清楚。为了预测未鉴定的小分泌蛋白的功能和生理作用，关于其表达模式的信息是有价值的。在这里，我们报告的拟南芥小分泌蛋白（ATSP）基因的表达分析，编码的蛋白质具有信号肽在N-末端，蛋白质的大小小于100个氨基酸残基。通过启动子：报告实验，我们研究了122个ATSPs的表达，包括47个未注释的ATSPs，没有任何可辨别的图案，在组织和细胞水平在拟南芥幼苗，花器官。结果表明，79个ATSP基因在幼苗的不同区域表达，其中37个ATSP基因特异性表达。
Small proteins secreted to the extracellular matrix in plants regulate many physiological activities, including pathogen response, material transport, and morphogenesis, but the functions of most small secreted proteins have not been elucidated except for some well-known small secreted proteins. To predict the functions and physiological roles of unidentified small secreted proteins, information on their expression patterns is valuable. Here, we report expression analysis of Arabidopsis thaliana small secreted protein (ATSP) genes that encode proteins possessing a signal peptide at N-terminal, and protein sizes were less than 100 amino acid residues. By promoter:reporter experiments, we examined the expression of 122 ATSPs, including 47 unannotated ATSPs that do not have any discernable motifs, in tissues and at the cellular level in Arabidopsis seedlings, and floral organs. As a result, 79 ATSP genes were expressed in various regions of the seedlings, and 37 ATSP genes were specifically expressed.