Morphological analysis of articular cartilage biopsies from a randomized. clinical study comparing the effects of 500-730 kDa sodium hyaluronate (Hyalgan®) and methylprednisolone acetate on primary osteoarthritis of the knee
Morphological analysis of articular cartilage biopsies from a randomized. clinical study comparing the effects of 500-730 kDa sodium hyaluronate (Hyalgan®) and methylprednisolone acetate on primary osteoarthritis of the knee
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DOI：
10.1053/joca.2000.0398
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发表时间：
2001-05-01
影响因子：
7
通讯作者：
Frizziero, L
中科院分区：
文献类型：
--
作者：
Guidolin, DD;Ronchetti, IP;Frizziero, L
目的：对关节内注射特定组分（500-730 kDa）透明质酸之前和之后6个月取自骨关节炎人膝关节的软骨样本进行组织形态计量学研究。与透明质酸获得的结果进行了比较与醋酸甲泼尼龙treatment.Methods的结果：24例原发性骨关节炎（OA）的膝盖被认为是。11名患者用Hyalgan（R），20 mg/2 ml，每周一次，持续5周）治疗，13名患者用甲泼尼龙（Depo-Medrol（R），40 mg/1 ml，每周一次，持续3周）治疗。在基线时和治疗开始6个月后，进行软骨活检并进行电子显微镜检查。关节表面形态，领土矩阵，软骨细胞数量和超微结构的特点是由一组形态计量学参数。样品从19个告知患者显示没有关节镜迹象OA也用于comparation.Results：透明质酸治疗后6个月的一个显着的重建的浅层观察到一起的改善软骨细胞密度和领土矩阵外观。此外，软骨细胞在其代谢方面出现显著改善，如通过合成结构和线粒体相对于具有分解代谢或储存功能的细胞器的增加的延伸所指示的。透明质酸治疗产生的结果是显着优于上级那些交付与甲基强的松龙在几乎所有的形态estimators.Conclusions：这些结果不能简单地解释由暂时恢复的滑液粘弹性，并提供进一步的证据表明，在这项研究中使用的透明质酸的具体部分是一个有用的工具，在OA治疗，具有潜在的结构修饰活性。(C)2001年国际骨关节炎研究学会。
Objective: Histomorphometric study on cartilage samples taken from osteoarthritic human knees before and 6 months after intraarticular injections of a specific fraction (500-730 kDa) of hyaluronan. The results obtained with hyaluronan were compared with the results of methylprednisolone acetate treatment.Methods: Twenty-four subjects with primary osteoarthritis (OA) of the knee were considered. Eleven patients were treated with Hyaluronan (Hyalgan(R), 20 mg/2 ml once a week for 5 weeks) and 13 with methylprednisolone (Depo-Medrol(R), 40 mg/1 ml once a week for 3 weeks). At the time of baseline and after 6 months from the start of treatment, biopsies of cartilage were taken and processed for electron microscopy. Articular surface morphology, territorial matrix, chondrocyte number and ultrastructure were characterized by a set of morphometric parameters. Samples from 19 informed patients showing no arthroscopic sign of OA were also used for comparison.Results: Six months after hyaluronan treatment a significant reconstitution of the superficial layer were observed together with an improvement in chondrocyte density and territorial matrix appearance. Furthermore, chondrocytes appeared significantly improved in their metabolism, as indicated by the increased extension of the synthetic structures and mitochondria with respect to the organelles having catabolic or storage functions. Hyaluronan treatment produced results that were significantly superior to those delivered with Methylprednisolone in almost all the morphometric estimators.Conclusions: These results cannot be explained simply by temporary restoration of the synovial fluid viscoelasticity, and provide further evidence that the specific fraction of hyaluronan used in this study is a useful tool in OA treatment, with a potential structure-modifying activity. (C) 2001 OsteoArthritis Research Society International.