Involvement of Streptococcus mutans regulator RR11 in oxidative stress response during biofilm growth and in the development of genetic competence.
Involvement of Streptococcus mutans regulator RR11 in oxidative stress response during biofilm growth and in the development of genetic competence.
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DOI：
10.1111/j.1472-765x.2008.02455.x
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发表时间：
2008-11
影响因子：
2.4
通讯作者：
Cvitkovitch DG
中科院分区：
文献类型：
--
作者：
Perry JA;Lévesque CM;Suntharaligam P;Mair RW;Bu M;Cline RT;Peterson SN;Cvitkovitch DG
鉴定变形链球菌HK/RR11双组分系统的调节因子RR11调控的基因。突变链球菌RR11编码基因被灭活，并检测了基因中断对细胞在应激条件下形成生物膜和获取胞外DNA能力的影响。缺乏RR11的细胞在暴露于氧化应激后生物膜减少。RR11-缺陷细胞显示约。转化效率降低20倍。用微阵列技术破译生物膜中受RR11调控的基因，结果显示大约。5%的UA159基因组发生了显著的表达变化。RR11被发现调控174个基因，包括与能力、应激反应和细胞分裂有关的基因。通过比较RR11缺陷突变株和亲本菌株的转录谱，确定了RR11在生物膜生长过程中控制的靶基因。结果表明，RR11参与了多种细胞过程的调控，包括氧化应激下生物膜的形成和遗传能力的发展。HK/RR11系统的调节子控制着一个大的调节子，是变形链球菌生物膜生长过程中参与应激反应的重要调节子，使其后代在微生物群落中得以生存和持续。
To identify the genes regulated by RR11, the regulator of the Streptococcus mutans HK/RR11 two-component system. The S. mutans RR11-encoding gene was inactivated, and the effects of gene disruption on the cell's ability to form biofilms under stresses and acquire extracellular DNA were tested. Biofilm was reduced in cells lacking RR11 following exposure to oxidative stress. RR11-defective cells showed approx. 20-fold reduction in transformation efficiency. Microarray used to decipher the RR11-regulated genes in biofilm showed that approx. 5% of the UA159 genome underwent a significant change in expression. RR11 was found to regulate 174 genes, including genes involved in competence, stress-response and cell division. Target genes controlled by RR11during biofilm growth have been identified by a comparison of transcriptional profiles between an RR11 defective mutant and the parental strain. The results demonstrated that RR11 is involved in the control of diverse cellular processes, including the formation of biofilm under oxidative stress and development of genetic competence. The regulator of HK/RR11 system controls a large regulon and is an important regulator involved in stress response during S. mutans biofilm growth enabling the survival and persistence of its progeny in the microbial community.