Skin flaps inhibit both the current of injury at the amputation surface and regeneration of that limb in newts
Skin flaps inhibit both the current of injury at the amputation surface and regeneration of that limb in newts
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DOI：
10.1002/jez.10141
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发表时间：
2002-10-01
影响因子：
--
通讯作者：
Borgens, RB
中科院分区：
文献类型：
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作者：
Altizer, AM;Stewart, SG;Borgens, RB
二十多年来，我们一直在研究一个强大的（ca。20-100 μ A/cm（2）），在再生蝾螈截肢后的最初几天，向外定向的电流通过肢体残端驱动。该电流由残肢完整皮肤的阿米洛利敏感的经皮电压在近端/远端方向上驱动通过残肢。肢体再生可以通过几种操纵这种生理学的技术来操纵，这表明离子电流对于两栖动物肢体的正常再生是必要的，但不是足够的。在这里，我们证明了覆盖蝾螈前肢残端的皮肤的全厚度移植物显著抑制肢体的再生，并且这个过程也与抑制那些不能再生的动物中的峰值向外定向的残端电流高度相关。(C)2002 Wiley-Liss，Inc.
For over two decades, we have been investigating a strong (ca. 20-100 muA/cm(2)), outwardly directed electric current driven through the limb stump for the first few days following amputation in regenerating salamanders. This current is driven through the stump in a proximal/distal direction by the amiloride-sensitive transcutaneous voltage of the intact skin of the stump. Limb regeneration can be manipulated by several techniques that manipulate this physiology, demonstrating that the ionic current is necessary, but not sufficient, for normal regeneration of the amphibian limb. Here, we demonstrate that a full thickness graft of skin covering the forelimb stump of newts strikingly inhibits the regeneration of the limb, and that this procedure is also highly correlated to a suppression of peak outwardly directed stump currents in those animals that fail to regenerate. (C) 2002 Wiley-Liss, Inc.