Sulfhydryl chemistry and solubility properties of human plasma apolipoprotein B.
Sulfhydryl chemistry and solubility properties of human plasma apolipoprotein B.
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人血浆载脂蛋白 B 的巯基化学和溶解度特性。
DOI：
10.1021/bi00261a048
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Jackson,RL
中科院分区：
文献类型：
--
作者：
Cardin,AD;Witt,KR;Barnhart,CL;Jackson,RL
艾伦·D作者声明：Kathleen R. Witt，Carole L. Barnhart，and Richard L.杰克逊 ** 摘要：从人血浆低密度脂蛋白（LDL; d=1.02-1.05 g/mL）中分离出载脂蛋白B（apo B）。在通过透析将去污剂减少至< 0.1 pg癸基硫酸钠/mg蛋白质后，接着用乙醇沉淀apoB以除去残余脂质，然后溶解在6 M盐酸胍（Gdn-HCl）中。apoB在6 M Gdn-HCl中对10 mM三（羟甲基）氨基甲烷盐酸盐（Tris-HCl）（pH 8.5）透析，导致蛋白质沉淀;然而，对6 M尿素透析，然后对10 mM Tris-HCl（pH 8.5）透析，导致水溶性载脂蛋白。水溶性载脂蛋白B给出了一个单一的沉淀线与LDL的完全身份时，测试抗载脂蛋白B的免疫扩散。
Alan D. Cardin, Kathleen R. Witt, Carole L. Barnhart, and Richard L. Jackson** abstract: Apolipoprotein B (apoB) was isolated from human plasma low-density lipoproteins (LDL; d=1.02-1.05 g/mL) by delipidation with ether-ethanol, followed by solubilization of the protein with sodium decyl sulfate. After the detergent was reduced to< 0.1 pg of sodium decyl sulfate/mg of protein by dialysis, apoB was next precipitatedwith ethanol to remove residual lipids and then solubilized in 6 M guanidine hydrochloride (Gdn-HCl). Dialysis of apoB in 6 M Gdn-HCl against 10 mM tris (hydroxymethyl) aminomethanehydrochloride (Tris-HCl), pH 8.5, resulted in protein precipitation; however, dialysis against 6 M urea and then 10 mM Tris-HCl, pH 8.5, resulted in a water-soluble apoprotein. Water-soluble apoB gave a single precipitin line of complete identity with LDL when tested by immunodiffusion against anti-apoB.