MALDI-TOF mass spectrometry of oligomeric food polyphenols
MALDI-TOF mass spectrometry of oligomeric food polyphenols
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DOI：
10.1016/j.phytochem.2005.05.015
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发表时间：
2005-09-01
期刊：
影响因子：
3.8
通讯作者：
Vestling, MM
中科院分区：
文献类型：
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作者：
Reed, JD;Krueger, CG;Vestling, MM
用MALDI-TOF MS对蔓越莓、葡萄籽提取物、高粱和石榴中多酚类物质进行了结构异质性表征，并以反式-3-吲哚丙烯酸为基质进行了MALDI-TOF MS分析。光谱分析提供了聚合程度、单体取代和分子间键性质的信息。蔓越莓聚黄烷-3-醇在A型和b型黄烷间键上存在差异，并且含有通过CH3-CH桥与花青素连接的聚黄烷-3-醇。葡萄籽提取物中聚没食子酰-聚黄烷-3-醇的没食子酰取代度变化较大。高粱多黄素在糖基化和羟基化上具有结构异质性。石榴可水解单宁与先前描述的结构相对应，如石榴金酸，但也观察到其他与低聚鞣皮单宁相对应的低聚鞣皮单宁，其中2至5个核心葡萄糖单元由脱氢二丙烯和/或valoneoyl单元交联。结果表明，在聚合程度、分子间键、羟基化模式以及与单糖和没食子酸的取代方面存在很大的异质性。(c) 2005 Elsevier Ltd版权所有。
The structural heterogeneity of polyphenols from cranberries, grape seed extracts, sorghum and pomegranate was characterized by MALDI-TOF MS. Polyphenolics were isolated by liquid chromatography and subjected to MALDI-TOF MS using trans-3-indoleacrylic acid as the matrix. Spectrometric analysis gave information on degree of polymerization, monomeric substitution, and the nature of intermolecular bonds. Cranberry polyflavan-3-ols had variation in interflavan bonds (A- and B-types) and contained polyflavan-3-ols linked to anthocyanins through a CH3-CH bridge. Polygalloyl-polyflavan-3-ols in grape seed extract had large variation in the degree of galloyl substitution. Sorghum polyflavans had structural heterogeneity in glycosylation and hydroxylation. Pomegranate hydrolyzable tannins that correspond to previously described structures were detected, such as punicalagin, but others that correspond to oligomeric ellgitannins in which two to five core glucose units are cross-linked by dehydrodigalloyl and or valoneoyl units were also observed. Results demonstrate that large heterogeneity occurs in degree of polymerization, intermolecular bonds, pattern of hydroxylation, and substitution with monosaccharides and gallic acid. (c) 2005 Elsevier Ltd. All rights reserved.