Plasma Dynamic Viscosity Determined by NMR
Plasma Dynamic Viscosity Determined by NMR
复制标题
通过 NMR 测定等离子体动态粘度
DOI：
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发表时间：
2018
影响因子：
1
通讯作者：
J. Philippé
中科院分区：
文献类型：
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作者：
M. A. Guevara;Yulianela Mengana Torres;J. C. G. Naranjo;N. Suárez;L. C. S. Beyríes;María A. Marichal Feliu;Teresa Simón Boada;Inocente C. Rodríguez Reyes;J. Philippé
本文提出了一种基于核磁共振（NMR）的测定血浆溶液动态粘度（η）的实验方法。考虑到血浆是白蛋白的极度稀释的水溶液，大分子之间没有长程流体力学相互作用，水分子在自由水和缔合水之间快速交换，得到了η与横向质子磁弛豫率（$$1/T2）的关系式。Carr-Purcell-Meiboom-Gill脉冲序列用于测量耦合到一个均匀磁系统（0.095 T）的磁共振控制台中的横向质子磁弛豫时间（T2）。在20个对照样品中获得的η值为1.68 ± 0.08 mPa s，其在统计学上与使用奥斯特瓦尔德粘度计在相同样品中获得的值（1.61 ± 0.04 mPa s）匹配。在172例多发性骨髓瘤患者（2.47 ± 0.15 mPa s）和72例镰状细胞病患者（2.45 ± 0.24 mPa s）中测定η，显示出与对照个体相比统计学显著增加。结果表明，这种NMR方法的实用性，估计动态粘度在血浆中的医疗目的，并与其他方法进行了比较。
A nuclear magnetic resonance (NMR) based experimental procedure to determine the dynamic viscosity (η) in blood plasma solutions is presented. An equation relating η and the transverse proton magnetic relaxation rate ($$1/T_{2}$$1/T2) is obtained after considering plasma an extremely diluted water solution of albumin with no long range hydrodynamics interactions among macromolecules, and a fast exchange of water molecules between the free and associated water. Carr–Purcell–Meiboom–Gill pulse sequence was used to measure the transverse proton magnetic relaxation time (T2) in a magnetic resonance console coupled to one homogeneous magnetic system (0.095 T). A η value of 1.68 ± 0.08 mPa s was obtained in 20 control samples, which statistically matched the value obtained in the same samples using an Ostwald viscometer (1.61 ± 0.04 mPa s). η was determined in 172 patients with multiple myeloma (2.47 ± 0.15 mPa s) and 72 with sickle cell disease (2.45 ± 0.24 mPa s) showing a statistically significant increase over the control individuals. The results show the utility of this NMR method to estimate dynamic viscosity in plasma for medical purposes, and a comparison with other methods is done.