VOLATILE PRODUCTS OF THE LIPOXYGENASE PATHWAY EVOLVED FROM PHASEOLUS-VULGARIS (L) LEAVES INOCULATED WITH PSEUDOMONAS-SYRINGAE PV-PHASEOLICOLA
VOLATILE PRODUCTS OF THE LIPOXYGENASE PATHWAY EVOLVED FROM PHASEOLUS-VULGARIS (L) LEAVES INOCULATED WITH PSEUDOMONAS-SYRINGAE PV-PHASEOLICOLA
复制标题
DOI：
10.1104/pp.101.1.13
复制
发表时间：
1993-01-01
期刊：
影响因子：
7.4
通讯作者：
SLUSARENKO, AJ
中科院分区：
文献类型：
--
作者：
CROFT, KPC;JUTTNER, F;SLUSARENKO, AJ
植物中“脂氧合酶途径”的激活产生一系列由脂肪酸衍生的产物。气相色谱-质谱法分析菜豆挥发性物质cv Red Mexican leaves在对植物病原性细菌Pseudomonaslagingae pv phaseolicola的过敏性抗性反应（HR）期间表现出几种脂质衍生的挥发物的进化，包括顺式-3-己烯醇和反式-2-己烯醛，其来自亚麻酸的13-氢过氧化物。这些化合物产生的缓冲液接种的叶子，也没有发展到如此高的程度，在可比阶段的敏感反应。反式-2，顺式-6-壬二烯醛，预期从9-氢过氧化的亚麻酸的产品的情况下，表明在HR期间的脂质过氧化主要是通过酶促豆脂氧合酶，产生的13-氢过氧化物，而不是通过自氧化过程。研究了反式-2-己烯醛、顺式-3-己烯醇和愈伤酸对菜豆条斑病菌的影响。反式-2-己烯醛在低浓度下表现出高度杀菌性，而顺式-3-己烯醇仅在高得多的浓度下才具有杀菌性。创伤酸似乎对Ps没有影响。PV.在测试的浓度下，这些结果表明，在植物对微生物攻击的防御反应过程中，植物产生了几种脂质衍生的化合物，其中一些具有抗微生物活性，可以想象参与植物抗病性。这些物质的产生时间，其量预计在体外具有抗菌作用，与叶片中细菌生长速率的减缓相关，并且在宿主中的植物抗毒素积累之前的时间被观察到。
Activation of the ''lipoxygenase pathway'' in plants gives rise to a series of products derived from fatty acids. Analysis by gas chromatography-mass spectroscopy of volatile products produced by Phaseolus vulgaris (L.) cv Red Mexican leaves during a hypersensitive resistance response (HR) to the plant pathogenic bacterium Pseudomonas syringae pv phaseolicola showed evolution of several lipid-derived volatiles, including cis-3-hexenol and trans-2-hexenal, which arise from the 13-hydroperoxide of linolenic acid. These compounds were not produced in detectable amounts by buffer-inoculated leaves, nor did they evolve to such a high degree during comparable stages of the susceptible response. The absence of trans-2,cis-6-nonadienal, a product expected from 9-hydroperoxide of linolenic acid, suggests that lipid peroxidation during the HR proceeded primarily enzymically via bean lipoxygenase, which produces the 13-hydroperoxide, and not via autoxidative processes. The effects of trans-2-hexenal, cis-3-hexenol, and traumatic acid on Ps pv phaseolicola were investigated. trans-2-Hexenal appeared to be highly bactericidal at low concentrations, whereas cis-3-hexenol was bactericidal only at much higher concentrations. Traumatic acid appeared to have no effect on Ps. pv. phaseolicola at the concentrations tested. These results demonstrate that during plant defense responses against microbial attack, several lipid-derived compounds are produced by the plant, some of which possess antimicrobial activity and conceivably are involved in plant disease resistance. The time of production of these substances, in amounts that would be expected to be antibacterial in vitro, correlated with a slowing down of the growth rate of bacteria in the leaves and was seen at a time before the accumulation of isoflavonoid phytoalexins in the host.