Membrane Protein Insertion into and Compatibility with Biomimetic Membranes
Membrane Protein Insertion into and Compatibility with Biomimetic Membranes
复制标题
膜蛋白插入仿生膜并与仿生膜相容
DOI：
10.1002/adbi.201700053
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发表时间：
2017
影响因子：
4.1
通讯作者：
Schantz, A. Benjamin
中科院分区：
文献类型：
--
作者：
Ren, Tingwei;Erbakan, Mustafa;Shen, Yuexiao;Barbieri, Eduardo;Saboe, Patrick;Feroz, Hasin;Yan, Hengjing;McCuskey, Samantha;Hall, Joseph F.;Schantz, A. Benjamin
膜蛋白和膜蛋白模拟功能化材料正迅速获得广泛应用的兴趣，包括药物筛选，DNA测序，药物输送，传感器，海水淡化和生物电子学。在这些应用中，材料性能高度依赖于双分子层和类双分子层结构（统称为仿生膜）中的每蛋白活性和蛋白质堆积密度。然而，尚不存在对仿生膜这些特性的清晰认识和准确的研究工具。本文介绍了评价膜蛋白与仿生膜材料相容性的方法。所使用的方法提供了平均单个蛋白质活性，并且首次提供了蛋白质（及其模拟物）与膜材料之间化学和物理相容性的实验量化测量。水转运蛋白、视紫红质和人工水通道被重组成目前的全范围仿生膜基质，以评估所提出的平台。相容性测试结果表明，生物和人工水通道在仿生膜中基本保持了单蛋白的水运输速率，但其重构密度不同，导致膜的整体透性不同。研究还表明，膜蛋白插入效率与膜蛋白与膜基质之间的化学和物理疏水性失配成反比。
Membrane protein and membrane protein–mimic functionalized materials are rapidly gaining interest across a wide range of applications, including drug screening, DNA sequencing, drug delivery, sensors, water desalination, and bioelectronics. In these applications, material performance is highly dependent on activity‐per‐protein and protein packing density in bilayer and bilayer‐like structures collectively known as biomimetic membranes. However, a clear understanding of, and accurate tools to study these properties of biomimetic membranes does not exist. This paper presents methods to evaluate membrane protein compatibility with biomimetic membrane materials. The methods utilized provide average single protein activity, and for the first time, provide experimentally quantifiable measures of the chemical and physical compatibility between proteins (and their mimics) and membrane materials. Water transport proteins, rhodopsins, and artificial water channels are reconstituted into the full range of current biomimetic membrane matrices to evaluate the proposed platform. Compatibility measurement results show that both biological and artificial water channels tested largely preserve their single protein water transport rates in biomimetic membranes, while their reconstitution density is variable, leading to different overall membrane permeabilities. It is also shown that membrane protein insertion efficiency inversely correlates with both chemical and physical hydrophobicity mismatch between membrane protein and the membrane matrix.
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影响因子：
9.5
作者：
Wang, Hong Lei;Chung, Tai-Shung;Hong, Minghui
通讯作者：
Hong, Minghui
DOI：
10.1074/jbc.m113.477893
发表时间：
2013-06-21
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Knyazev DG;Lents A;Krause E;Ollinger N;Siligan C;Papinski D;Winter L;Horner A;Pohl P
通讯作者：
Pohl P
影响因子：
2.9
作者：
KAPLAN, RS;PEDERSEN, PL
通讯作者：
PEDERSEN, PL
影响因子：
5.5
作者：
Bermudez, H;Brannan, AK;Discher, DE
通讯作者：
Discher, DE
影响因子：
2.4
作者：
VANHEESWIJK, MPE;VANOS, CH
通讯作者：
VANOS, CH